UPLC快速分析穿心莲药材及制剂中内酯成分  

UPLC Determination of Lactone in Andrographis paniculata Preparation

作　　者：黄朋纳[1] 

机构地区：[1]广州市医药职业学校,广州510430

出　　处：《中国实验方剂学杂志》2012年第14期98-101,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立UPLC-PDA技术快速测定穿心莲药材及制剂中主要内酯类成分含量的方法。方法:采用超高效液相色谱仪,用Acquity BEH Shield RP C18色谱柱(2.1 mm×50 mm,1.7μm);流动相甲醇(A)-水(B),梯度洗脱(0～2 min,40%A;2～4 min,40%～45%A;4～10 min,45%～50%A;10～15 min,50%A);流速0.5 mL.min-1,检测波长226 nm,柱温30℃。结果:在15 min内可以完成药材和制剂中穿心莲内酯、脱水穿心莲内酯的分析,分离度良好,线性范围分别为0.028 6～0.286 g.L-1(r=0.999 6,n=6),0.094 0～0.940 g.L-1(r=0.999 3,n=6);平均加样回收率分别为97.32%,97.11%。结论:该方法快速、准确、灵敏度高,可用于穿心莲药材及制剂的质量控制。Objective：To develop an UPLC method for the Determination of lactone in Andrographis paniculata peparation.Method： Acquity BEH Shield RP C18 column（2.1 mm×50 mm,1.7 μm） was used with Methanol（A）-Water（B） as a mobile phase by gradient elution program（0-2 min,40%A;2-4 min,40%-45% A;4-10 min,45%-50% A;10-15 min,50% A）.The flow rate was 0.5 mL·min-1.The detecting wavelength was 226 nm.The column temperature was 30 ℃.Result： The linear ranges of andrographolide and dehydroandrographoline were 0.028 6-0.286 g·L-1（r=0.999 6,n=6）,0.094 0-0.940 g·L-1（r=0.999 3,n=6）.The average recoveries were 97.32% and 97.11%.Conclusion： The method was accurate,quickly and sensitive.It can be used for quality control of A.paniculata peparation.

关 键 词：穿心莲 超高液相色谱法 穿心莲内酯 脱水穿心莲内酯 

分 类 号：R284.1[医药卫生—中药学]