白鲜皮中白鲜碱的分离及抗炎活性  

Isolation and Anti-inflammatory Effect of Dictamnine Extracted from Dictamni Cortex

作　　者：时东方[1] 郑梅竹[1] 赵立春[2] 张红晶 李丽[1] 刘春明[1] 

机构地区：[1]长春师范学院,长春130032 [2]广西中医学院附属瑞康医院,南宁530011 [3]长春迪瑞医疗科技有限公司,长春130012

出　　处：《中国实验方剂学杂志》2012年第14期128-131,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省发改委项目[吉发改高技(2010)362号];吉林省教育厅项目[吉教科合字(2012)第225号]

摘　　要：目的:提取分离白鲜碱单体,对白鲜皮醇提物及白鲜碱单体进行抗炎效果检测。方法:采用高速逆流色谱分离的方法分离得到白鲜碱单体;利用小鼠耳廓肿胀及小鼠腹腔毛细血管渗透性实验确定待试物抗炎活性。结果:选择正己烷-乙酸乙酯-乙醇-水(1∶1∶1∶1)溶剂系统对白鲜皮醇提物进行分离,经HPLC检测,所分离白鲜碱纯度达98%。白鲜皮粗提物的各剂量组可显著抑制由二甲苯引起的小鼠耳廓肿胀,抑制率分别为44.26%,52.46%,44.26%,差异显著(P<0.05);白鲜碱中剂量组对小鼠耳廓肿胀亦有抑制作用,抑制率为25.00%,且差异显著(P<0.01);但在抑制醋酸致小鼠腹腔毛细血管渗透性增高方面,低剂量组效果不明显。结论:高速逆流色谱分离白鲜碱,简单快速易于操作,且具有较好的分离效果;白鲜皮醇提物及白鲜碱单体具有很好的抗炎作用,不同抗炎模型对抗炎效果表现出一定的差异性。Objective：To detect the anti-inflammatory effects of ethanol extracts and dictamnine extracted and isolated from Dictamni Cortex.Method：High performance centrifugal partition chromatography method was used for separation of the essential dictamnine,and anti-inflammatory effects of ethanol extracts and dictamnine were investigated by xylene-induced aruical swelling in mice and the abdominal capillary permeability in mice.Result：Separation was performed with a two-phase solvent system composed of hexane ethylacetate-ethanol-water（1∶1∶1∶1）.The purity of dictamnine obtained was 98% determined by high performance liquid chromatograph.The results showed that each dosage of ethanol extracts could significantly（P0.05） suppress the mouse auricle which caused by the xylene swelling（being 44.26%,52.46%,44.26%,respectively）,as compared with the control group.The medium-dosage group of dictamnine could obviously（P0.01） suppress the mouse auricle but the dosage was not increased significantly in abdominal capillary permeability in mice compared with control group.Conclusion： HPCPC is a recommendable method to prepare and purify the dictamnie with good separation and the method is simple,accurate and easy to operate.The results showed that ethanol extracts and dictamnine showed different anti-inflammatory effect in variety of animal models of anti-inflammatory.

关 键 词：白鲜皮 白鲜碱 高速逆流色谱 分离 抗炎 

分 类 号：R284.1[医药卫生—中药学]