冰片对栀子在小鼠体内药代动力学的影响  

Effects of Borneol on Pharmacokintics of Geniposide of Gardenia Jasminoides in Mice

作　　者：田秀峰[1] 李鹏跃[2] 王宏洁[2] 边宝林[2] 杜守颖[1] 

机构地区：[1]北京中医药大学,北京100102 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2012年第14期135-138,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项基金(20092X09502-008);国家自然基金面上项目(81073057);北京中医药大学复方中药制药创新团队(2011-CX70-13);教育部博士点基金(20090013110007)

摘　　要：目的:建立小鼠血浆中栀子苷的高效液相色谱(HPLC)分析方法,研究冰片对栀子苷在小鼠体内药代动力学的影响。方法:小鼠分别口服给予栀子提取物及栀子提取物加冰片,采用HPLC法测定小鼠血浆中栀子苷浓度,以Kinetica软件拟合,计算相关药动学参数。结果:栀子苷在血浆中1.188～76.000 mg.L-1线性良好(r=0.999 3,n=5)。栀子单独给药,栀子-冰片配伍给药栀子苷的代谢均符合非房室模型。栀子与冰片配伍给药可以使栀子苷血药浓度(Cmax)增大,药-时曲线下面积(AUC)明显增加,血药浓度平均滞留时间(MRT)延长。结论:冰片与栀子配伍应用能提高栀子苷的吸收,延长其消除,提高生物利用度。Objective：To establish a method for determination of geniposide in mice plasma by high performance liquid chromatography（HPLC）,and study the effect of borneol on the pharmacokinetics of geniposide in mice with the proposed method.Method： Mice were divided into two groups and administrated with the extraction of Gardenia jasminoides and the extraction coupled with Borneol,respectively.HPLC was used for the determination of geniposide in plasma,and the pharmacokinetic parameters were calculated by the software of Kinetica.Result： The good linearity range of geniposide in plasma was 1.187 5-76 mg · L-1（r=0.999 3,n=5）.The pharmacokinetics model of geniposide in the two groups followed the non-compartment model.Compared with the group administrated with gardenia,the gardenia-borneol group showed significant increase in the area under the plasma concentration-time curve（AUC） and in the peak concentration（Cmax）,with an extension of the mean residence time（MRT）.Conclusion： Borneol could accelerate the absorption of geniposide,decelerate its clearance speed,increase its bioavailability.

关 键 词：栀子 栀子苷 冰片 药代动力学 配伍应用 

分 类 号：R285.5[医药卫生—中药学]