全蝎-蜈蚣对CIA大鼠外周血协同刺激分子B7-1,B7-2表达的影响  

Express of B7-1 and B7-2 in Peripheral Blood from Rats with Collagen-Induced Arthritis Treated by Scorpio-Scolopendra

作　　者：黄小英[1] 赵海梅[2] 左志琴[2] 程绍民[3] 刘端勇[2] 

机构地区：[1]江西中医学院科研处,南昌330004 [2]江西中医学院科技学院,南昌330025 [3]江西中医学院基础医学院,南昌330004

出　　处：《中国实验方剂学杂志》2012年第14期151-154,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30860377);江西省自然科学基金项目(2009GZY0118);江西省卫生厅科技计划(20092052)

摘　　要：目的:观察全蝎-蜈蚣对胶原免疫性关节炎(collagen-induced arthritis,CIA)大鼠外周血协同刺激分子B7-1(CD80)、B7-2(CD86)表达的影响。方法:Wistar大白鼠60只,随机分成6组,即正常组,模型组,全蝎-蜈蚣高,中,低剂量组,Ⅱ型胶原蛋白(typeⅡcollagen,CⅡ)组。采用Ⅱ型胶原蛋白诱导法复制大鼠关节炎模型,并ig给予全蝎-蜈蚣混悬液(0.4,0.2,0.1 g.kg-1)进行干预,CⅡ组予CⅡ白冻干粉60μg.kg-1ig进行对照,共计40 d。并采用流式细胞术检测大鼠外周血单核细胞表达CD80,CD86情况。结果:与正常组大鼠外周血CD80+(44.8±11.61)%CD80+CD86-(32.4±9.18)%比较,模型组大鼠外周血CD80+细胞(55.7±6.31)%及CD80+CD86-细胞(43.7±10.26)%水平显著升高(P<0.05);与模型组比较,全蝎-蜈蚣中、低剂量组大鼠外周血CD80+细胞(46.6±6.34)%,(30.8±15.14)%明显降低(P<0.05),及且全蝎-蜈蚣低剂量组大鼠外周血CD80+CD86-细胞(25.3±12.67)%水平显著下降(P<0.05)。结论:全蝎-蜈蚣药对治疗CIA大鼠的作用机制可能与降低外周血单核细胞表达CD80+及CD80+CD86-分子表达水平相关。Objective：To observe express of B7-1（CD80） and B7-2（CD86） in peripheral blood from rats with collagen-induced arthritis（CIA） treated by Scorpio-Scolopendra.Method： Sixty female Wistar rats were randomly divided into 6 groups,the normal control group,the model control group,the low-dose,the middle-dose and the high-dose Scorpio-Scolopendra group（1∶1）,and the type Ⅱcollagen group.Rat arthritis model was induced by injection of collagen.The number of CD80＋ and CD86＋cell in peripheral blood was tested by flow cytometry.Result： Compared with the model groups,the level of CD80＋ cell（55.7±6.31）% was decreased obviously in the middle and low dose of Scorpio-Scolopendra groups（46.6±6.34,30.8±15.14）%（P0.05）.While the level of the CD80＋CD86-cell（43.7±10.26）%in peripheral blood from rats in the model groups was higher than in the low dose of Scorpio and Scolopendra group（25.3±12.67）%（P0.05）.Conclusion： The mechanism of Scorpio and Scolopendra treated RA is likely to down-regulate express of B7-1（CD80） and B7-2（CD86） in peripheral blood.

关 键 词：全蝎 蜈蚣 类风湿性关节炎 B7-1 B7-2 

分 类 号：R285.5[医药卫生—中药学]