淫羊藿素对雌激素依赖性乳腺癌MCF-7细胞作用的影响  

The Role of Icaritin on Estrogen-dependent Breast Cancer Cell MCF-7

作　　者：胡蕾蕾[1] 段小群[1] 卢曦[1] 李军[2] 张可锋[1] 孙晓玲[1] 李云秋[1] 

机构地区：[1]桂林医学院,广西桂林541004 [2]新加坡国立大学999002

出　　处：《中国实验方剂学杂志》2012年第14期155-158,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81060272);广西医学科学实验中心开发课题(KFJJ2011-12)

摘　　要：目的:探讨淫羊藿素(ICT)对雌激素依赖性乳腺癌MCF-7细胞的作用。方法::以在37℃、5%CO2培养箱培养的人乳腺癌MCF-7为研究对象,用不同浓度的淫羊藿素单独作用及与雌二醇联合作用,测定人乳腺癌MCF-7细胞的增殖率,并检测该过程中雌激素受体ERα的表达情况。结果:当ICT浓度大于1×10-10mol.L-1时单独使用能促进ERα阳性人乳腺癌MCF-7细胞增殖,能诱导ERα基因的表达;淫羊藿素与雌二醇联合作用抑制MCF-7细胞增殖,抑制ERα的表达。结论:淫羊藿素与雌二醇联合作用具有抑制E2诱导的人乳腺癌MCF-7细胞增殖作用;淫羊藿素与雌二醇联合作用可抑制ERα基因的表达。Objective：To observe the role of icaritin on Estrogen-dependent breast cancer cell line MCF-7.Method： MCF-7 human breast cancer was used to observe proliferation rate by different concentrations of icaritin alone and combined effects of estrogen in human breast cancer MCF-7 cell,and test the expression of estrogen receptor（ERα）.Result： When MCF-7 cells were treated with ICT,MCF-7 cells proliferation was promoted.The activity of ERα report gene expression could be induced by icaritin.The effect of estradiol（E2） could be antagonized by icaritin when treated with the combined icaritin and E2.Conclusion： The icaritin can promote MCF-7 cells proliferation and has certain phytoestrogenic effects.The activity of ERα report gene expression can be antagonized by icaritin.

关 键 词：淫羊藿素 人乳腺癌细胞MCF-7 雌激素 雌激素受体ERα 

分 类 号：R285.5[医药卫生—中药学]