槐定碱对急性肺损伤小鼠肺组织SOD,MDA及TLR4表达的影响  

Effect of Sophoridine on Expression of Superoxide Dismutase,Malonaldehyde and TLR4 mRNA in Acute Lung Injury Mice Induced by Lipopolysaccharide

作　　者：杨翠萍[1] 杨晓金[2] 田真真[1] 叶荷平[1] 朱金华[1] 付艳丽[1] 万红娇[1] 

机构地区：[1]江西中医学院,南昌330004 [2]南昌大学医学院2010级硕士研究生,南昌330006

出　　处：《中国实验方剂学杂志》2012年第14期180-183,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省教育厅青年科学基金项目(GJJ11190)

摘　　要：目的:研究槐定碱对内毒素(LPS)致急性肺损伤(ALI)小鼠肺组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及Toll样受体4(TLR4)表达的影响。方法:采用随机数字法,将60只清洁级昆明种小鼠分成6组:正常组、模型组、预防给药组、槐定碱低剂量组、槐定碱中剂量组、槐定碱高剂量组,每组10只。①正常组、②模型组、④槐定碱低剂量组、⑤槐定碱中剂量组、⑥槐定碱高剂量组:ip生理盐水0.5 mL,连续6 d。③预防给药组:ip 5 mg.kg-1槐定碱,连续6 d。上述②～⑥组均在末次注射生理盐水或药物后1 h ip脂多糖(LPS)9 mg.kg-1造模,造模2 h后④～⑥组分别给槐定碱9,5,2.5 mg.kg-1。6 h后检测肺组织匀浆中SOD活性及MDA的含量,取新鲜肺组织裂解后提取RNA,RT-PCR法检测TLR4 mRNA的表达水平。结果:与正常组(86.22±8.86)U.mg-1相比,LPS模型组(50.58±10.06)U.mg-1肺组织匀浆SOD活性明显下降;与模型组相比,各给药组SOD[预防给药组(64.71±5.74)U.mg-1,高剂量组(56.34±6.88)U.mg-1,中剂量组(75.50±13.01)U.mg-1,低剂量组(67.02±10.19)U.mg-1]均明显升高(P<0.05);而肺组织中MDA含量变化趋势与SOD相反(P<0.05);TLR4 mRNA的表达灰度值与正常组(0.45±0.20)比较,LPS模型组(0.82±0.20)明显升高;与模型组比较,各用药组[预防给药组(0.60±0.32)s,高剂量(0.55±0.19),中剂量(0.54±0.29),低剂量组(0.61±0.26)]均明显降低(P<0.05)。结论:槐定碱能降低ALI模型小鼠肺组织匀浆中MDA的活性,提高SOD含量,降低TLR4 mRNA的表达。Objective：To study the effect of Sophoridine on expression of super oxide dismutase（SOD）,malonaldehyde（MDA）and Toll-like receptor 4（TLR4）of in acute lung injury（ALI） mice induced by lipopolysaccharide（LPS）.Method： Sixty healthy KM mice were randomly divided into 6 groups： normal group,model group,prevention treatment group,sophoridine high dose group,sophoridine middle dose group,low dose group sophoridine,10 mice each group.The normal group,model group,sophoridine high,middle and low groups were lavaged 0.5 mL sodium chloride and the prevention treatment group was lavaged 0.5 mL sophoridine every day.It lasted 6 days.The disposable intraperitoneal injection of endotoxin method was used to establish intends to induce acute lung injury model in mice.The model was set up in the sixth day by injecting LPS 9 mg·kg-1.2 hours later,sophoridine high,middle and low groups were given 9,5,2.5 mg·kg-1 respectively.According to the manual of kits,SOD,MDA were detected.Reverse transcriptase-PCR was used to study the expression of TLR4 mRNA.Result： In model group SOD content（50.58±10.06） compared to other groups decreased significantly（P0.05）.In model group MDA content（2.01±0.15） and the expression of TLR4 mRNA compared to other groups increased significantly（P0.05）.Conclusion： Sophoridine can reduce ALI model mice lung tissue MDA content and the expression of TLR4 mRNA and increase the value of SOD.

关 键 词：槐定碱 急性肺损伤 脂多糖 超氧化物歧化酶 丙二醛 TOLL样受体 

分 类 号：R285.5[医药卫生—中药学]