激光等离子体效应对细胞烧蚀特性研究  

Research on Cells Ablation Characters by Laser Plasma

作　　者：韩敬华[1] 张新刚[2] 蔡晓唐[3] 段涛[4] 冯国英[1] 杨李茗[5] 张亚筠[1] 王绍朋[1] 李世文[1] 

机构地区：[1]四川大学电子信息学院,四川成都610064 [2]四川大学生命科学学院,四川成都610064 [3]四川大学华西第二医院,四川成都610041 [4]西南科技大学极端条件物质特性实验室,四川绵阳621010 [5]成都精密光学工程研究中心,四川成都610041

出　　处：《光谱学与光谱分析》2012年第8期2022-2026,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金重大项目(60890203);西南科技大学极端条件物质特性实验室开放基金项目(11zxjk08)资助

摘　　要：研究对比了激光直接辐照、聚焦辐照以及激光等离子体辐照三种辐照方式下,洋葱表皮细胞的烧蚀特征,并基于激光辐照的热力学特性对细胞的温升以及相变过程进行分析。观察发现:直接辐照对细胞的杀伤效果很不明显;聚焦辐照会引起焦点附近细胞的断裂以及脱水;激光等离子体辐照作用下,细胞会呈现大面积的去除,断裂边缘粗糙,且细胞层有叠加现象。理论分析发现,激光等离子体具有热效应、辐射电离及冲击波效应等,会增加激光脉冲能量到细胞的沉积、以及对细胞冲击剥离等,从而会大大增加细胞的杀伤范围和效率,可用于对细胞进行大面积杀伤。The study on the mechanism of laser ablated ceils is of importance to laser surgery and killing harmful cells. Three ra- diation modes were researched on the ablation characteristics of onion epidermal cells under： laser direct irradiation, focused irra-diation and the laser plasma radiation. Based on the thermodynamic properties of the laser irradiation, the cell temperature rise and phase change have been analyzed. The experiments show that the cells damage under direct irradiation is not obvious at all, but the focused irradiation can cause cells to split and moisture removal. The removal shape is circular with larger area and rough fracture edges. The theoretical analysis found out that the laser plasma effects play a key role in the laser ablation. The thermal effects, radiation ionization and shock waves can increase the deposition of laser pulses energy and impact peeling of the ceils, which will greatly increase the scope and efficiency of cell killing and is suitable for the cell destruction.

关 键 词：激光等离子体 细胞烧蚀 辐射电离 冲击波 

分 类 号：Q233[生物学—细胞生物学] O433.4[机械工程—光学工程]