不同中药复方对慢性心衰大鼠MMP-2、TIMP-2影响的实验研究  

Experiment Research on Effect of Different Chinese Medicine Compound on MMP-2 and TIMP-2 Expressions in Rats with Chronic Heart Failure

作　　者：张艳[1] 胡晓红[2] 廖佳丹[2] 王彩玲[2] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]辽宁中医药大学,辽宁沈阳110032

出　　处：《辽宁中医杂志》2012年第7期1205-1207,I0001,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：沈阳市科技局应用基础研究(F10-205-1-68)

摘　　要：目的:通过研究基质金属蛋白酶(MMP-2)及内源性基质金属蛋白酶组织抑制剂(TIMP-2)在慢性心衰中的作用,从分子生物学角度探讨中药不同配伍对慢性心衰心室重构的影响,并进一步阐明其作用机理。方法:本实验以冠脉结扎法配合力竭式游泳、减食等方法造成大鼠慢性心衰动物模型,分成模型组、西药组、益气中药组、活血中药组、益气活血中药组、益气活血利水中药组和正常组。利用实时荧光定量PCR技术及SABC免疫组化法检测慢性心衰大鼠MMP-2、TIMP-2表达的变化情况。结果:慢性心衰动物模型组大鼠心肌MMP-2表达明显升高,TIMP-2表达降低,与假手术组比较,差异有统计学意义(P<0.01);经过治疗,各用药组大鼠心肌组织MMP-2表达明显降低,TIMP-2表达升高,其中益气活血组、益气活血利水组中药与西药组比较,差异无统计学意义(P>0.05),且益气活血利水组疗效明显优于益气活血组(P<0.01)。结论:证明益气活血利水中药可通过作用于心肌组织中MMP-2、TIMP-2的表达,抑制或逆转心室重构的过程,从而达到治疗慢性心衰的目的。Objective：By investigating the effects of different Chinese medicine compounds on expressions of matrix metallopeptidase 2（MMP-2）and tissue inhibitor of metalloproteinase-2（TIMP-2）in chronic heart failure（CHF）,we studied the effect of traditional Chinese medicine on further mechanism of ventricular remodeling in CHF on the role of molecular biological sense.Methods：The coronary artery ligation method with exhaustion swimming and reduce feeding was used to cause chronic heart failure rats model.Rats were divided into model group,west medicine control group,Qi-supplementing group,blood-activating group,Qi-supplementing and blood-activating group,Yiqi huoxue Lishui treatment group and normal group.The real-time quantitative PCR technique and SABC immunohistochemical method were used to detect MMP-2 and TIMP-2 changes of CHF rats.Results：MMP-2 expression in animal model of chronic heart failure group increased obviously,and TIMP-2 expression decreased,compared with the surgical group,and differences were statistically significant（P〈0.01）.After treatment,MMP-2 expression in the medication group significantly reduced obviously,and TIMP-2 expression increased.The difference in the supplementing Qi and activating blood group,Yiqi huoxue Lishui treatment group and Western medicine group were no statistically significant（P〈0.05）.And therapeutic effect of Yiqi huoxue Lishui group was much better than that in the supplementing Qi and activating blood group（P〈0.01）.Conclusion：It is proved Chinese compound of supplementing Qi,activating blood circulation and promoting urination can inhibit or reverse ventricular remodeling processon by acting on MMP-2 and TIMP-2 in organizations,so as to achieve the goal of therapy for chronic heart failure.

关 键 词：益气活血利水 实时荧光定量PCR 免疫组化法 MMP-9 

分 类 号：R285.5[医药卫生—中药学]