RP-HPLC法同时测定冠心丹参胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量  

RP-HPLC Simultaneous Dermination of Notoginsenoside R1、Ginsenoside Rg1 and Ginsenoside Rb1 in GuanXin Danshan Capsule

作　　者：罗镭[1] 唐登峰[1] 祝明[1] 

机构地区：[1]浙江省食品药品检验所,浙江杭州310004

出　　处：《亚太传统医药》2012年第7期14-16,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:建立高效液相色谱法同时测定冠心丹参中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的方法。方法:采用Kromasil-C18(250mm×4.6mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱,流速1.0mL.min-1,柱温23℃,检测波长203nm。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1进样量分别在0.0655～1.092μg/mL(r=0.9998),0.1980～3.2997μg/mL(r=0.9998),0.1802～3.0032μg/mL(r=0.9999)范围内与峰面积呈良好线性关系;平均回收率分别为98.8%,100.3%,100.4%,RSD均小于3%。结论:所建立的方法操作简便,测定结果准确,重复性好,可用于冠心丹参胶囊的质量控制。Objective:To establish an HPLC method for simultaneous determination of Notoginsenoside R1、Ginsenoside Rg1 and Ginsenoside Rb1 in GuanXin Danshan Capsule.Methods:The chromatographic separation was chieved on Kromasil-C18(250mm×4.6mm,5μm)column with acetonitrile-water as mobile phase(gradient elution).The flow rate was 1.0 mL·min-1,the column temperature was maintained at 23℃,and the detective wavelength was set at 203nm.Results:The calibration curves of Notoginsenoside R1、Ginsenoside Rg1 and Ginsenoside Rb1 were linear in the ranges of 0.0655~1.092μg(r=0.9998),0.1980~3.2997μg(r=0.9998),0.1802~3.0032μg(r=0.9999)repentively.The average recoveries were 98.8% 100.3 % and 100.4%,respectively,with RSD less than 3%.Conclusion:The method is simple,rapid,accurate and reproducible for quanlity control of GuanXin Danshan Capsule.

关 键 词：冠心丹参胶囊 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 反相高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]