不同部位伤害性刺激下大鼠脑功能性磁共振成像的变化  

Changes in brain functional magnetic resonance imaging during noxious electric stimulation of forepaw and tail in rats

作　　者：傅小云[1] 谢鹏[2] 涂业[2] 喻田[2] 吕粟[3] 龚启勇[3] 

机构地区：[1]遵义医学院附属医院重症医学科,563003 [2]遵义医学院麻醉学系 [3]四川大学华西医院磁共振研究中心

出　　处：《中华麻醉学杂志》2012年第6期670-672,共3页Chinese Journal of Anesthesiology

基　　金：贵州省社会发展攻关计划（[2007]1034）

摘　　要：目的观察不同部位伤害性刺激下大鼠脑功能性磁共振成像的变化。方法健康清洁级SD大鼠，体重300-450g，雌雄不拘，采用Y型迷宫筛选出对电刺激反应较敏感的48只大鼠，采用随机数定表法，将其随机分为2组（n=24）：刺激鼠尾组（T组）和刺激左前爪组（LF组）。T组于鼠尾给予伤害性电刺激，LF组于左前爪给予伤害性电刺激，刺激期间行脑功能磁共振成像扫描，采用统计参数图2软件行图像分析。结果T组中枢主要的激活脑区包括：初级感觉皮层、次级感觉皮质、后扣带回皮质、杏仁核和腹后外侧丘脑核；LF组中枢主要的激活脑区包括：右侧伏膈核、右侧初级感觉皮层、右侧腹后外侧丘脑核和右侧后扣带回皮质。结论不同部位伤害性刺激下大鼠脑功能性磁共振成像在中枢激活的脑区不同。Objective To investigate the change in brain functional magnetic resonance imaging （fMRI） during noxious electric stimulation of forepaw and tail in rats. Methods Forty-eight healthy SD rats of both sexes weighing 300-450 g which responded sensitively to electrical stimulation as shown by Y-maze were randomly divided into 2 groups （ n = 24 each） receiving nociceptive electric stimulation of left forepaw （group LF） and tail （group T） respectively. The animals were anesthetized with intraperitoneal 10% chloral hydrate 250-300 mg/kg. Brain fMRI was performed at 30 min after disappearance of righting reflex in both groups when electric stimulations were delivered intermittently to forepaw or tail. The images were analyzed with SPM2 software. Results Primary and secondary somatosensory cortex, posterior cingutate cortex, amygdaloid nucleus and ventral posterolateral thalamic nucleus were activated in group T. Right accumbens nucleus, right primary somatosensory cortex, right ventral posterolateral thalamic nucleus and right posterior cingutate cortex were activated in group LF. Conclusion Different brain areas are activated by noxious electric stimulation of different parts of body as shown by brain fMRI.

关 键 词：物理刺激 磁共振成像 脑 

分 类 号：R445.2[医药卫生—影像医学与核医学]