UFLC法同时测定参芎葡萄糖注射液中6种主要成分  

Simultaneous determination of six essential components in Shenxiong Glucose Injection by UFLC

作　　者：郑林[1,2] 庞秀清[1,2] 兰燕宇[1,2] 李勇军[1,2] 王永林[1,2] 黄勇[1,2] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004 [2]贵州省药物制剂重点实验室,贵州贵阳550004

出　　处：《中成药》2012年第7期1276-1279,共4页Chinese Traditional Patent Medicine

基　　金：贵州省中药现代化科技产业专项项目(黔科合社字[2009]5008号);贵州省科技计划项目(黔科合重大专项字[2011]6019号);贵阳市科技计划项目([2010]筑科农合同字第1-11号)

摘　　要：目的建立超快速液相色谱(UFLC)同时测定参芎葡萄糖注射液(丹参、盐酸川芎嗪)中丹参素、盐酸川芎嗪、原儿茶醛、迷迭香酸、丹酚酸B、丹酚酸A的方法。方法采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05%磷酸为流动相进行梯度洗脱,体积流量为1 mL/min,检测波长220 nm,柱温40℃。结果 6种被测成分的线性关系良好(r>0.999),精密度、重复性、稳定性的RSD都低于3%,平均加样回收率在95.19%～101.02%,RSD在0.92%～2.70%(n=6)。结论本方法简便、快速、准确,能有效控制参芎葡萄糖注射液的质量。AIM To establish a UFLC method for determining tanshinol,tetramethylpyrazine hydrochloride,protocatechuic aldehyde,rosmarinic acid B and salvianolic acid B,salvianolic acid A in Shenxiong Glucose Injection（Salviae miltiorrhizae Radix et Rhizoma,tetramethylpyrazine hydrochloride）.METHODS Chromatogaphic analysis was performed on Diamonsil C18 column（4.6 mm×250 mm,5 μm） with acetonitrile-0.05% phosphoric acid as mobile phase in gradient elution mode.The flow rate was 1 mL/min with the detection wavelength of 220 nm.The column temperature was set at 40 ℃.RESULTS All six measured components showed good linearity（r0.999） in the ranges of the test concentration.RSDs of the precision,reproducibility and stability test were less than 3%.The average recoveries of the method were in the ranges of 95.19%-101.02%,RSDs were in the ranges of 0.92%-2.70%（n=6）.CONCLUSION The method is simple,rapid and accurate,could be used for the quality control of Shenxiong Glucose Injection.

关 键 词：超快速液相色谱 参芎葡萄糖注射液 丹参素 盐酸川芎嗪 原儿茶醛 迷迭香酸 丹酚酸B 丹酚酸A 

分 类 号：R927.2[医药卫生—药学]