鸦胆子油HPLC-ELSD指纹图谱研究  

Fingerprint analysis of Brucea javanica oil by HPLC-ELSD

作　　者：郭辉[1,2] 胡晨[2] 钱俊青[2] 李宏 岳昌林 

机构地区：[1]浙江九旭药业有限公司,浙江金华321016 [2]浙江工业大学药学院,浙江杭州310014

出　　处：《中成药》2012年第7期1311-1315,共5页Chinese Traditional Patent Medicine

摘　　要：目的建立鸦胆子油的HPLC-ELSD指纹图谱分析方法。方法采用Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-异丙醇-正己烷;采用蒸发光检测器。采用《中药色谱指纹图谱相似度评价》(2004 A版)软件,对10批不同批次的鸦胆子指纹图谱进行相似度计算。并采用液相色谱-质谱联用(HPLC-MS)方法对鸦胆子油中的化学成分进行定性分析。结果首次建立了鸦胆子油HLPC-ELSD特征指纹图谱共有模式,共标定了9个共有峰,通过液相色谱-质谱联用方法指认了其中8个共有峰,10批鸦胆子油的相似度为0.996～1。结论该方法具有良好的精密度,稳定性和重复性,可用于鸦胆子油的质量控制评价。AIM To establish HPLC-ELSD fingerprint for the quality control of Brucea javanica oil.METHODS Analysis on an Agilent Eclipse XDB-C18（4.6 mm×250 mm,5 μm） column with an evaporative light-scattering detector and eluted with mobile phases containing acetonitrile-isopropanol-hexane.And data of ten batches of Brucea javanica samples from different sources were analysed by ＂Similarity Evaluation for Chromatographic Fingerprint of Traditional Chinese Medicine＂ software in combination with HPLC-MS.RESULTS Nine common peaks were selected in chromatograms,eight of them were identified.The similarity of ten batches of Brucea javanical oil was 0.991-1.CONCLUSION The precision,reproducibility and stability of this method are satisfying,and it can be used to identify and evaluate the quality of Brucea javanica oil.

关 键 词：鸦胆子油 指纹图谱 HPLC-ELSD 

分 类 号：R284.1[医药卫生—中药学]