同步辐射圆二色谱法研究Fe_3O_4纳米颗粒对细胞色素C结构的影响  

Effects of Fe_3O_4 nanoparticles on secondary structure of cytochrome c by synchrotron radiation circular dichroism spectroscopy

作　　者：魏慧芳[1,2] 汪冰[2] 高迪[1,2] 关铭[1] 陈汉清[2] 蒋静[2] 屈秀云[2] 郑令娜[2] 欧阳宏[2] 丰伟悦[2] 

机构地区：[1]吉林大学药学院,长春130022 [2]中国科学院高能物理研究所核分析技术院重点实验室及纳米生物效应与安全性院重点实验室,北京100049

出　　处：《核技术》2012年第7期491-494,共4页Nuclear Techniques

基　　金：国家自然科学基金(10905064;10975148)资助

摘　　要：四氧化三铁纳米颗粒(Fe3O4-NPs)在生物医学领域有着广泛的应用。通过同步辐射真空紫外圆二色谱(SRCD)和紫外可见(UV-Vis)吸收谱技术研究了Fe3O4纳米颗粒的粒径(10 nm和40 nm)对细胞色素C(Cyt c)结构的影响。结果表明,随着Fe3O4-NPs颗粒物浓度的增大,Cyt c在408 nm处的吸光度值逐渐下降。SRCD结果显示Cyt c与Fe3O4-NPs作用后,蛋白的α螺旋含量减少,β折叠含量增加;Fe3O4-NPs与Cyt C的作用强度具有尺寸依赖性,小尺寸的Fe3O4-NPs作用更为明显。Supermagnetic iron oxide nanoparticles （Fe3O4-NPs） can promote many attractive functions in biomedical fields. In this study, the interaction of Fe3O4NPs （10 nm and 40 nm） with cytochrome c （Cyt c） was studied by synchrotron radiation circular dichroism （SRCD） and UV-Vis spectroscopy. After adding Fe3O4 NPs in the Cyt c solution, the intensity of the Soret band （408 nm） decreased significantly. The SRCD characterization showed the loss of the α-helix structure and the occurrence of cytochrome c unfolding after Fe3O3-NPs treatment, meanwhile, the 10 nm Fe3O3-NPs had more prominent effects than the 40 nm ones. The results showed significant size-depended and dose-depended effects. This study provides important insight into the interaction of Fe3O4 NPs with cytochrome c, which may be a useful guideline for further use of Fe3O4 NPs.

关 键 词：四氧化三铁纳米颗粒 细胞色素C 同步辐射圆二色谱 

分 类 号：Q71[生物学—分子生物学]