人参皂苷对DC2.4吞噬抗原和表达CD40分子的影响  

Influence of Ginsenoside on Phagocytosis and CD40 Expression of DC2.4

作　　者：殷胜骏[1,2] 彭桂英[2] 顾立刚[2] 韩涛[2] 周英武[2] 万巧凤[2] 

机构地区：[1]天津医科大学第二医院,天津300211 [2]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药学刊》2012年第7期1487-1489,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金资助项目(30701105);北京中医药大学自主选题项目(2009JYBZZ-XS016)

摘　　要：目的:观察人参皂苷Rg1、Rb1及其联合使用对树突状细胞系DC2.4吞噬抗原和表面分子表达的影响。方法:体外培养DC2.4细胞,分别加入不同剂量人参皂苷Rg1、Rb1及Rg1+Rb1孵育24h后,分别加入异硫氰酸荧光素标记的卵白蛋白(FITC-OVA),流式细胞仪检测DC2.4细胞吞噬卵白蛋白抗原的情况;体外培养DC2.4细胞,分别加入LPS和不同剂量人参皂苷孵育24h,流式细胞仪检测细胞表面分子CD40的表达。结果:人参皂苷Rg1、Rb1、Rg1+Rb1在一定浓度可促进DC2.4细胞抗原吞噬的能力;三者均抑制CD40的表达。结论:人参皂苷Rg1、Rb1可通过影响DCs的抗原吞噬功能来发挥免疫调节作用;Rg1、Rb1联合使用与单独应用比较无明显差异。Objective：To observe the influence of ginsenoside Rg1,Rb1 and their combined use on phagocytosis and expression of surface molecules of DC2.4 cells.Methods：DC2.4 cells were cultured in vitro.The cells were treated by varying concentrations of ginsenosides Rg1,Rb1 and Rg1＋Rb1 for 24h,and then FITC-OVA was involved or not.The phagocytosis and expression of the surface molecules CD40 were detected by flow cytometry.Results：The low and high dose of Rg1,Rb1 and Rg1＋Rb1 promoted the phagocytosis.However,they all inhibited the expression of CD40.Conclusion：Rg1,Rb1 and their combined use had an influence on phagocytosis of DC2.4 cells,which regulated immune function.The combined use of them failed to reinforce their effect.

关 键 词：人参皂苷 DC2.4细胞 抗原吞噬 表面分子 

分 类 号：R285.5[医药卫生—中药学]