芍药甘草汤有效组分对慢性坐骨神经结扎大鼠机械痛敏和热痛敏的影响  

Influence of Shaoyao Gancao Decoction Effective Ingredients on Mechanica Lallodynia and Heat Hyperalgesia Induced by Chronic Constriction Injury of the Sciatic Nerve in Rats

作　　者：张娟[1] 郑晖[1] 郭小文[1] 王海娜[2] 

机构地区：[1]浙江省中医院,浙江杭州310006 [2]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2012年第7期1602-1604,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2009YA005)

摘　　要：目的:观察芍药甘草汤有效组分(SGM)对慢性压迫性损伤(CCI)大鼠机械痛敏和热痛敏的影响,并研究SGM的给药时机和是否有超前镇痛作用。方法:所有大鼠用机械缩足反射阈值(MWT)和热缩足潜伏期(TWL)来分别评价大鼠机械痛敏和热痛敏。SD大鼠随机分为9组,即假手术组、CCI组和空白组,BN组和BS组,于术前7天开始灌胃给予0.9%生理盐水或SGM120mg/kg,每天2次,直至手术当天结束;AN组、AS组为后给药组,于术后第1天给予直至术后第7天;BAN组和BAS组术前7天前开始直至术后第7天。各组分别在术前、术后1、3、7天分别测定MWT和TWL。结果:和空白组和假手术组相比,CCI组术后MWT及TWL明显下降(P<0.01)。BS、AS和BAS组与各生理盐水组相比,MWT和TWL在术后1、3和7天均明显增加(P<0.01)。BS组和AS组相比,术后第1天的MWT和TWL均明显增加(P<0.01),但到术后第7天,AS组的MWT和TWL均高于BS组(P<0.01),BAS组和AS组相比,术后第1天的MWT和TWL均明显增加(P<0.01),但到术后第7天,两者无明显差异。结论:CCI大鼠从术后1天形成稳定的机械痛敏和热痛敏,SGM能抑制CCI大鼠的机械痛敏和热痛敏作用,术前给予SGM对NP有超前镇痛作用,镇痛作用并能持续数天。和术后用药相比,术前后给药组镇痛作用出现更快。Objective：To observe the effect and the preemptive analgesia of the extracts of Shaoyao Gancao Decoction（SGM）on mechanical allodynia and heat hyperalgesia induced by chronic constriction injury（CCI）of the sciatic nerve in rats.Methods：Rats were divided into nine groups.Mechanical allodynia and heat hyperalgesia were evaluated by mechanical withdrawal threshold（MWT）and thermalwithdrawal latency（TWL）respectively.In a preemptive treatment groups（BN and BS groups）,normal saline（2μL）and SGM（120mg/kg）were orally administered on 7th day before surgery;postoperative treatment groups（AN and AS groups）were administered on 7th day after surgery,pre-postoperative treatment groups（BAN and BAS groups）were administered on 7th day before surgery to 7th day after surgery.All groups were administered twice a day.The TWL and MWT were measured before surgery and on 1st,3rd and 7th day after surgery.Results：PWL and PWT of rats with CCI had a significantly decrease from the first day after surgery to the end of this study（P〈0.01）.MWT and TWL in BS and AS and BAS groups evidently increased（P〈0.01）.MWT and TWL in BS group evidently increased on 1st day（P〈0.01）,but the result was contrary on 7 days after surgery in comparison to AS group（P〈0.01）.Compared to AS group,MWT and TWL in BAS group evidently increased on 1st day（P〈0.01）,but there was no difference on 7 days after surgery.Conclusion： CCI group formed steady mechanical allodynia and heat hyperalgeia from the first day after surgery to the end of this study.SGM orally administered evidently increased MWT and TWL of the CCI rats.Preemptive treatment with SGM remarkably brought the preemptive analgesia and the effects would continue for several days.Compared to AS group,BAS group affected faster for the analgesia.

关 键 词：芍药 甘草 神经病理性疼痛 超前镇痛 

分 类 号：R285.5[医药卫生—中药学]