新疆甜高粱种质资源遗传多样性的SSR分析  

Simple Sequence Repeats Polymorphism Analysis of Sweet Sorghum Germplasm in Xinjiang

作　　者：冯国郡[1,2] 叶凯[2] 李桂英[3] 聂元冬[3] 郭建富[2] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业科学院,乌鲁木齐830091 [3]中国农科院作物科学研究所,北京100081

出　　处：《植物遗传资源学报》2012年第4期549-554,561,共7页Journal of Plant Genetic Resources

基　　金：新疆自然科学基金项目(2010211A60);现代高粱产业技术体系建设项目(nycytx-12)

摘　　要：选用50对SSR引物对新疆现有72份甜高粱种质资源进行遗传多样性分析。结果表明:有20对引物在72份甜高粱种质资源中表现为多态性。共检测到91个等位基因,每对引物可检测到的等位基因数目为2～5个,平均为3.45个。多态性信息量(PIC)的变动范围为0.2859～0.6652,平均为0.5057。72份甜高粱种质间的遗传相似系数变化范围为0.2001～1.000,平均值为0.5599。UPGMA聚类分析将72份材料划分为A、B两大类群,A群包括69份材料,而A群又被分成从Ⅰ到Ⅺ共11个亚群,B群包括3份材料,农艺性状近似的大多被聚到同一类群。Totally,72 sweet sorghum germplasm in Xinjiang were used to analyze their genetic diversity and genetic relationship using 50 SSR primers.The result indicated that a total of 20 SSR primers performed polymorphism and 91 alleles loci were detected.The average number of alleles per SSR was 3.45 with a range from 2 to 5.The value of allelic polymorphic information content（PIC） ranged from 0.2859 to 0.6652,on an average of 0.5057 per SSR marker.The genetic similarity coefficient ranged from 0.2001 to 1.000 with a average of 0.5599.The cluster analysis grouped the 72 sweet sorghum cultivars and lines into 2 main groups.Group A included 69 accessions and group B included only 3 accessions.Group A was again sub-divided into sub-groups I through XI.The accessions of sweet sorghum with similar agronomic trait was mostly clustered one group.

关 键 词：SSR 甜高粱 遗传多样性 遗传距离 聚类分析 

分 类 号：S514[农业科学—作物学]