小麦高温抗条锈性表达与蛋白质合成的关系  

Expression of High Temperature Resistance to Stripe Rust and Protein Synthesis in Wheat

作　　者：商鸿生[1] 张慧 李振岐 

机构地区：[1]西北农林科技大学植物保护系,陕西杨陵712100

出　　处：《麦类作物》2000年第1期16-19,共4页

基　　金：高等学校博士学科点专项科研基金!(910710;970702)

摘　　要：小麦高温抗条锈性是一种在相对高温下表达的非小种专化抗病性，以典型高温抗条锈品种小偃６号为试材，研究了高温抗条锈性表达与蛋白质合成的关系。小偃 ６ 号小麦叶片接种条锈菌（Ｐｕｃｃｉｎｉａｓｔｒｉｉｆｏｒｍ ｉｓ）ＣＹ２９ 小种后，常温（１４～１６ ℃）培养，寄主呈亲和反应，显症后移入高温（２１～２２ ℃）下继续培养，诱导了高温抗锈性，表现不亲和反应。蛋白质体内合成试验发现接种苗移入高温下处理２４ ｈ 后，接种叶中标记氨基酸掺入率分别增至未经高温处理的接种叶的１．５１ 倍和高温处理的未接种叶的１．４２ 倍。Ｐｏｌｙ（Ａ＋ ）－ＲＮＡ 体外翻译进一步证明高温处理导致接种叶出现编码６ 种新多肽的ｍ ＲＮＡ，其分子量分别为６９．８，４７．４４，４４．６，４１．７，４０．７ 和３５．５ ｋＤ，表明高温抗条锈性的表达与基因活化有关。High temperature resistance of wheat to stripe rust ( Puccinia striiformis West.) was a kind of race non specific resistance , which gave expression only at relatively high temperature . Xiao yan No.6, a typical cultivar with high temperature resistance , was used to reveal the relation between protein synthesis in vivo and resistance expression . Seedlings of Xiao yan No.6 inoculated with race CY29, showed compatible reaction when incubated at normal temperature (14～16℃),but changed to incompatible reaction after transferring to higher temperature (21～22℃) of incubation at the stage of symptom appearance . It was found by the experiment of protein in vivo synthesis that the in corporation of labeled amino acid in infected leaves in high temperature increased to 1.51 times of that in infected leaves in normal temperature, and to 1.42 times of that in uninoculated control in higher temperature respectively . The results of in vitro translation of Poly (A +) RNA showed that six mRNAs , which encoded polypeptides with molecular weight 69.8,47.4,44.6,41.7,40.7 and 35.5 kD respectively , were induced by high temperature treatment. The expression of high temperature resistance , therefore , was associated with gene activation in host.

关 键 词：小麦条锈病 高温抗病性 蛋白质合成 基因表达 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]