低氧化还原电位对铜绿微囊藻(Microcystis aeruginosa)生长的抑制及恢复的影响  

Growth inhibition and recovery from low oxidation reduction potential to Microcystis aeruginosa

作　　者：王健[1] 周天艺[1] 高文程[1] 浦海清[1] 杨柳燕[1,2] 

机构地区：[1]南京大学环境学院,南京210046 [2]污染控制与资源化研究国家重点实验室,南京210046

出　　处：《湖泊科学》2012年第4期528-534,共7页Journal of Lake Sciences

基　　金：国家重点基础研究发展计划"973"项目(2008CB418102);环保公益性行业科研专项项目(200809145);南京大学大学生创新训练计划项目联合资助

摘　　要：在实验室光照黑暗交替培养下,用半胱氨酸下调黑暗起始时的氧化还原电位(ORP),研究低电位对铜绿微囊藻(Microcystis aeruginosa FACHB 469)生长的影响,并探索低电位胁迫消除后铜绿微囊藻生长的恢复潜力.结果表明,在-50～-100 mV范围内水体ORP越低,其对铜绿微囊藻生长的抑制作用越强.低ORP可抑制铜绿微囊藻对磷的吸收,且这种抑制程度与电位变化无关.-50～-75 mV的电位对铜绿微囊藻SOD酶活性有促进作用,而-90～-100 mV电位明显抑制SOD酶活.当低ORP胁迫消除后,铜绿微囊藻能够恢复至最初的生长状态,同时伴随含磷量的提高和SOD酶活的恢复,-50～-75 mV电位胁迫对藻消除胁迫后的生长更有促进作用.因此,低ORP对铜绿微囊藻的生长会产生不利影响,但是该影响是可逆的.In this experiment, cysteine was used as an Oxidation Reduction Potential （ORP） adjustment to explore the responses of Microcystis aeruginosa （FACHB 469 ） under different ORPs＇ stimulation. A recovery experiment was also designed with M. aeruginosa separated from the former experiment and cultured in fresh medium. Results showed a negative effect of low ORP stimu- lation on the growth of M. aeruginosa. Within the range of -50 to - 100 mV, inhibition effect was intensified with the ORP＇ s decline. The uptake rate of phosphorus decreased under low ORP＇ s stress but had no relationship with stress strength. A proper low ORP＇ s stimulation （e. g. -50 and -75 mY） could intensify the SOD enzyme activity of M. aeruginosa, while over low （e. g. -90 and - 100 mV） had negative effects. M. aeruginosa could recover its growing ability after removing the lower ORP＇ s stimu- lation. The uptake rate of phosphorus and the SOD enzyme activity returned to normal as well Moreover, the enhancement of growth ability was observed by a low ORP＇ s stimulation （e.g. -50 and -75 mV）. Thus, the low ORP＇ s stimulation had nega- tive effect on the growth of M. aeruginosa, however the process can be reversible. K

关 键 词：铜绿微囊藻 氧化还原电位 生长抑制 恢复 磷 SOD酶 叶绿素A 

分 类 号：X524[环境科学与工程—环境工程]