兴仁金线莲丛生芽诱导增殖研究  

Study on the Induction and Proliferation of Clustered Buds of Anoectochilus xingrensis

作　　者：罗晓青[1] 蒙秋伊[1] 查兰松[1] 张志勇[1] 卢加举[1] 

机构地区：[1]贵州省亚热带作物研究所,贵州兴义562400

出　　处：《安徽农业科学》2012年第22期11231-11232,11260,共3页Journal of Anhui Agricultural Sciences

基　　金：贵州省农业攻关"贵州金线莲有机生产关键技术研究与加工应用"黔科合NY字[2010]3032号;贵州农科院专项"金线莲试管苗培育及人工种植关键技术研究"黔农科院专项[2009]013号;"贵州生物质能源植物研发创新能力建设"黔科合院所创能[2010]4007

摘　　要：[目的]筛选兴仁金线莲丛生芽的诱导和增殖的最佳培养基。[方法]用野生兴仁金线莲植株茎段作外植体,以MS为基本培养基,并添加不同浓度的6-BA和NAA,诱导丛生芽,筛选最佳培养基配方。[结果]MS是兴仁金线莲快繁最佳基本培养基;2.0～3.0 mg/L的6-BA有利于丛生芽的诱导;1.0 mg/L 6-BA+0.1 mg/L NAA有利于芽的生长;最佳蔗糖浓度为25 g/L。[结论]MS+2 mg/L 6-BA+0.2 mg/L NAA+25 g/L蔗糖为丛生芽增殖的最佳培养基配方。[Objective] To screen out the best medium for the induction and proliferation of A.xingrensi.[Method] The stem of wild A.xingrensi was used as explants to be cultured on the MS basic culture medium dosed with different concentration of 6-BA and NAA to induce clustered buds for quick proliferation.[Result] MS was the best culture medium.2.0-3.0 mg/L 6-BA could improve the induction of clustered buds.1.0 mg/L 6-BA together with 0.1 mg/L NAA could promote elongation of the buds.The best sucrose concentration was 25 g/L.[Conclusion] The best culture medium for proliferation of clustered buds was MS＋2 mg/L 6-BA＋0.2 mg/L NAA＋25 g/L sucrose.

关 键 词：兴仁金线莲(Anoectochilu sxingrensis) 组织培养 丛生芽 快繁 

分 类 号：S567[农业科学—中草药栽培]