叶绿素荧光仪检测重金属离子毒性的研究  

Research on the Toxicity of Heavy Metal Ions with Chlorophyll Fluorometer

作　　者：邹叶娜[1,2] 蔡焕兴 薛银刚 徐东炯 

机构地区：[1]常州大学环境与安全工程学院,江苏常州213164 [2]江苏省环境保护水环境生物监测重点实验室常州市环境监测中心,江苏常州213001

出　　处：《安徽农业科学》2012年第22期11375-11376,11413,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立一种快速有效的重金属离子毒性检测方法。[方法]选择三角褐指藻(Phaeodactylum tricornutum)为受试生物,采用双通道脉冲振幅调制叶绿素荧光仪(简称ToxY-PAM)检测水中Cu2+、Zn2+、Cr6+3种重金属离子的毒性大小。[结果]三者不同浓度引起的三角褐指藻光合作用抑制率变化基本均在1 h内达到最大值并趋于平稳;3种重金属离子的毒性大小为Cu2+>Zn2+>Cr6+。[结论]ToxY-PAM体积小,携带方便,检测速度快,灵敏度高,适合于重金属离子的现场检测。[Objective] The research aimed to establish a fast and effective methods about the detection of heavy metal ions toxicity.[Method]Choosing Phaeodactylum tricornutum as testing organisms,and using Dual-channel PAM Chlorophyll Fluorometer to detect Cu^2＋、Zn^2＋、Cr^6＋ in the water.[Result]The changes of photosynthesis inhibition rate caused by different concentrations of the three heavy metal ions almost reached maximum and stabilized in one hour;The order of toxicity intensity among the three heavy metal ions was as below： Cu^2＋Zn^2＋Cr^6＋.[Conclusion] ToxY-PAM instrument has small size and high sensitivity,convenient to carry,and the speed of detection is quickly,so it＇s suitable for detection of heavy metal ion in the field.

关 键 词：重金属离子 三角褐指藻 叶绿素荧光仪 生物毒性 

分 类 号：S181.3[农业科学—农业基础科学]