Preparation and cytocompatibility of polylactic acid/hydroxyapatite/graphene oxide nanocomposite fibrous membrane  

作　　者：MA HaiBin SU WenXin TAI ZhiXin SUN DongFei YAN XingBin LIU Bin XUE QunJi 

机构地区：[1]State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China [2]School of Stomatology, Lanzhou University, Lanzhou 730000, China [3]Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China

出　　处：《Chinese Science Bulletin》2012年第23期3051-3058,共8页

基　　金：supported by the Hundred Talents Program of Chinese Academy of Sciences and the National Natural Science Foundation of China(51005225)

摘　　要：A series of polylactic acid (PLA) based nanocomposite fibrous membranes,including neat PLA,PLA/hydroxyapatite (HA) and PLA/HA/graphene oxide (GO),were fabricated via electrospinning method.The morphology and composition were investigated by scanning electron microscopy (SEM),X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) respectively.The thermal stability was determined by thermogravimetric analysis (TGA).To estimate the cytocompatibility of asprepared PLA/HA/GO fibrous membrane,MC3T3-E1 cells were cultured,and the corresponding cell adhesion and differentiation capability were investigated by fluorescence microscopy,SEM and MTT test.The electrospun ternary PLA/HA/GO membrane exhibited three-dimensional fibrous structure with relatively rough surface morphology,which made itself ideal for cell attachment and proliferation in bone tissue regeneration.The fluorescence microscopy,SEM and MTT test confirmed that the PLA/HA/GO nanocomposite fibrous membrane created a proper environment for the seeding and proliferation of MC3T3-E1 cells.A series of polylactic acid （PLA） based nanocomposite fibrous membranes, including neat PLA, PLA/hydroxyapatite （HA） and PLA/HA/graphene oxide （GO）, were fabricated via electrospinning method. The morphology and composition were investigated by scanning electron microscopy （SEM）, X-ray diffraction （XRD） and Fourier transform infrared spectroscopy （FTIR） respectively. The thermal stability was determined by thermogravimetric analysis （TGA）. To estimate the cytocompatibility of asprepared PLA/HA/GO fibrous membrane, MC3T3-E1 cells were cultured, and the corresponding cell adhesion and differentiation capability were investigated by fluorescence microscopy, SEM and MTT test. The electrospun ternary PLA/HA/GO membrane exhibited three-dimensional fibrous structure with relatively rough surface morphology, which made itself ideal for cell attachment and proliferation in bone tissue regeneration. The fluorescence microscopy, SEM and MTT test confirmed that the PLA/HA/ GO nanocomposite fibrous membrane created a proper environment for the seeding and proliferation of MC3T3-E1 cells.

关 键 词：polylactic acid HYDROXYAPATITE graphene OXIDE FIBROUS membranes 

分 类 号：TB383.2[一般工业技术—材料科学与工程]