鱼油脂肪乳对肠外营养大鼠小肠黏膜紧密连接形态结构的影响  

Effects of fish oil emulsion on intestinal tight junction structure in rats which accepted total parenteral nutrition

作　　者：王萌[1] 汪灏[1] 袁献温[1] 管文贤[1] 

机构地区：[1]南京大学医学院附属鼓楼医院普通外科,江苏南京210008

出　　处：《肠外与肠内营养》2012年第4期223-227,共5页Parenteral & Enteral Nutrition

基　　金：黎介寿院士肠屏障专项研究基金资助(LJS_201012)

摘　　要：目的:探讨鱼油脂肪乳剂(富含ω-3多不饱和脂肪酸)对肠外营养(PN)大鼠肠上皮细胞紧密连接(TJ)形态和紧密连接蛋白occludin表达的影响。方法:将大鼠随机分为四组,即对照组(正常喂食),PN(禁食5d行PN支持)组,小剂量鱼油治疗(1 ml/kg鱼油静脉注射+PN)组和大剂量鱼油治疗(2 ml/kg鱼油静脉注射+PN)组。观察大鼠术后小肠黏膜上皮细胞TJ形态,以及紧密连接蛋白occludin的表达分布。结果:PN组大鼠治疗5 d后肠上皮细胞TJ结构部分缺失、间隙增宽;大剂量鱼油治疗组TJ形态基本完整,小剂量鱼油治疗组TJ部分缺失。各组occludin蛋白总量无明显差异。提取脂筏组分,发现与对照组比,三个实验组脂筏区域中occludin蛋白表达均明显减少,但大剂量鱼油治疗组较PN组明显升高(P<0.01)。结论:长期PN可导致TJ结构的缺失,脂筏区域紧密连接蛋白occludin表达减少。大剂量鱼油脂肪乳剂可维持occludin蛋白在脂筏区域的表达,保护小肠黏膜细胞TJ的结构和功能。Objective： To investigate the effect of fish oil emulsion on tight junction structure（TJ） and tight junction protein occludin expression in rats which accepted total parenteral nutrition（TPN） for 5 days.Methods： 32 SD rats were ramdomly divided into four groups（n=8）： control group,TPN group,TPN＋fish oil（1 ml/kg） group and TPN＋fish oil（2 ml/kg） group.The structure of tight junction was observed by transmission electric microscope.The expression of tight junction protein occludin was detected by Western Blot analysis.Results： Intestinal tight junction structure revealed morphological disruption after TPN for 5 days,and mostly restored by fish oil（2 ml/kg）.During this period,total amounts of TJ protein occludin remained unchanged.However,occludin expression in raft microdomains reduced significantly after TPN,fish oil（2 ml/kg） prevented occludin reduction in raft microdomains.Conclusion： Fish oil emulsion could protect intestinal tight junction structure in rats during TPN period,which was partly attributed to its modulation on occludin expression in raft microdomains.

关 键 词：Ω-3多不饱和脂肪酸 紧密连接 肠外营养 脂筏 

分 类 号：R459.3[医药卫生—治疗学]