跑台训练对脊髓挫伤大鼠后肢运动功能及去甲肾上腺素能纤维的影响  

Effects of treadmill training on noradrenaline fibers and the recovery of hindlimb motor function in rats after spinal cord contusion

作　　者：朱文秀[1] 周培[2] 吴东[1] 邹莉[1] 朱文倩 叶超群[4] 

机构地区：[1]临沂市人民医院,临沂276000 [2]首都体育学院 [3]沂南县人民医院普外科 [4]北京军区总医院骨科

出　　处：《中华物理医学与康复杂志》2012年第7期488-492,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：国家自然科学基金项目（81171862）

摘　　要：目的观察跑台训练对脊髓挫伤大鼠后肢运动功能及去甲肾上腺素能纤维的影响。方法将3月龄雌性SD大鼠制成脊髓中度挫伤模型，采用随机数字表法将其分为实验组及对照组。实验组大鼠于脊髓挫伤1周后给予跑台训练，对照组不给予任何特殊处理。于术后每周采用BBB评分及平均联合评分（ACOS）对各组大鼠后肢运动功能进行评定；于术后第9周时取各组大鼠受损脊髓制成标本行苏木精-伊红（HE）染色及免疫组织化学染色。结果术后第9周时2组大鼠后肢运动功能均有一定程度改善，其中实验组大鼠BBB评分达（15．38±3．62）分，ACOS评分达（78．00±10．39）分，与对照组间差异均具有统计学意义（P〈0．05）。实验组大鼠脊髓损伤尾侧胶质细胞及液化区数量均明显少于对照组；实验组大鼠受损脊髓多巴胺-β-羟化酶（DBH）阳性纤维计数为（17．75±2．22）根／每高倍镜视野，对照组大鼠DBH阳性纤维计数为（8．25±2．50）根／每高倍镜视野，组间差异具有统计学意义（P〈0．05）。实验组大鼠受损脊髓DBH阳性神经元计数为（25．25±7．76）个／每高倍镜视野，对照组大鼠DBH阳性神经元计数为（10．75±4．57）个／每高倍镜视野，组间差异具有统计学意义（P〈0．05）。结论跑台训练能促进脊髓挫伤大鼠后肢运动功能改善，其治疗机制可能与增加受损脊髓DBH阳性纤维及DBH阳性神经元数量有关。Objective To observe the effects of treadmill training on hindlimb motor recovery and noradrenaline （NA） fibers in rats with spinal cord contusion. Methods Spinal cord contusions at the T10 level were induced in 3-month old female SD rats by dropping a 10 g rod from a height of 25 mm. The injured rats were then randomly divided into a control group and a treadmill training group （ n = 15 in each group）. Treadmill training began 1 week post-injury. No treatment was administered to the control group rats. Hindlimb motor function was evaluated weekly from the 1st to the 9th week post-injury using Basso Beattie Bresnahan （BBB） scores and average combined scores （ACOs）. The expression of dopamine beta hydroxylase （DBH） in the spinal cord caudal to the injured area was detected by immunohistochemical staining. Results The treadmill training group exhibited significantly greater improvement in BBB scores from the 3rd week to the 7th week and in ACOs from the 2rid to the 9th week post-injury compared with the control group. The number of DBH positive fibers in the injured spinal cords and the number of DBI-I positive neurons in the injured frontal horns were also significantly higher in the training group. Conclusions Treadmill training can promote the recovery of hindlimb motor function in rats after spinal cord contusion, perhaps by increasing the number of DBH positive fibers and DBH positive neurons.

关 键 词：脊髓挫伤 跑台训练 去甲肾上腺素 

分 类 号：R651.2[医药卫生—外科学]