促进脂肪移植的最佳SVFs浓度的实验研究  

Experimental study on the best concentration of SVFs for promoting survival rate of fat graft

作　　者：朱茗[1] 鲁峰[1] 高建华[1] 廖云君[1] 

机构地区：[1]南方医科大学南方医院整形外科,广州510515

出　　处：《中华整形外科杂志》2012年第4期284-290,共7页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金资助项目（30901566）;国家自然科学基金资助项目（81071589）;广东省自然科学基金资助项目（9151051501000025）

摘　　要：目的探讨应用自人脂肪组织新鲜分离的不同浓度的血管基质层细胞（stromalvasvularfractioncells，SVFs）辅助脂肪移植，从而提高移植物的存活率的方法。方法脂肪组织来自临床行吸脂术者，提取SVFs，将0．3ml待移植的脂肪颗粒分别与浓度为①5×10^5个／ml（A组）；②1×10^6个／ml（B组）；③2×10^6个／ml（c组）的SVFs混合，另设对照组为完全培养基＋单纯脂肪颗粒（D组），随机注射移植于6只裸鼠背部皮下。术后3个月观察移植物情况：①湿重。②切片HE染色计数血管密度。③方网测试系统“点计数”法检测存活脂肪细胞计数以及纤维坏死组织计数。结果①湿重：A组（60．000±6．325）mg，B组（81．670±7．528）mg，C组（68．330±7．528）mg，D组（48．330±7．528）mg，B组脂肪存活率均高于A、C、D组（P〈0．05），A、C2组之间比较差异无统计学意义（P〉0．05）。②血管密度：A、B、C组血管密度均高于D组，且B组明显高于其他3组（P〈0．05），A、C2组之间比较差异无统计学意义（P〉0．05）。③点计数：B组存活脂肪细胞计数均高于A、C、D组（P〈0．05），纤维组织计数均低于13组（P〈0．05），A、C2组之间比较差异均无统计学意义（P〉0．05）。结论来源于人自体SVFs复合脂肪颗粒能够显著提高移植脂肪组织的成活率，其中1×10^6个／ml数量级的SVFs移植脂肪的存活率最高，更适合用于脂肪移植，具有广阔的临床应用前景。Objective To investigate the effect of different concentrations of human adipose stromal vascular fraction cells（SVFs） on the survival rate of fat transplantation. Methods 0. 3 ml fat tissue, derived and refined from clinical liposuction patients, was mixed with different concentrations of SVFs as 5 ×105^/ml in Group A, or 1 ×10^6/ml in Group B, or 2 ×10^6/ml in Group C, or completely medium in control group D. Then the mixture was injected randomly under the back skin of 6 nude mice. The transplanted fat tissue in four groups was harvested at 3 months after implantation. Wet weight of fat grafts was measured for macroscopic aspects. After HE staining, blood vessel density, viable adipocytes and fibrous proliferation were counted respectively for histological evaluation. Results The wet weight of fat grafts in group B （81. 670 ± 7. 528 ） mg was significantly higher than that in group A, C, D [ （60. 000 ± 6. 325） mg,（68.330±7.528） mg,（48,330±7.528） rag, respectively, P 〈0.05）],but the difference between group A and group C was not statistically significant（P 〉0.05）. The grafts in group A,B and C had significantly higher blood vessel density than those in the control group D,whereas blood vessel density was the highest in group B （ P 〈 0. 05 ） and there was no significant difference between group A and C （ P 〉 0.05）. Compared with group A, C and D, histological analysis revealed that the fat grafts in group B was consisted predominantly of adipose tissue with less fat necrosis and fibrosis （ P 〈 0.05）. However, fibrosis counts were significant lower in group A, B and C than those in group D （ P 〈 0.05） , and there was no significant difference between group A and C （P 〉 0. 05）. Conclusions The human isolated SVFs has the advantages to improve the survival rate of fat transplantation, and the magnitude of 1 × 106^/ml is more practical and safe,indicating a wide clinical application in the future.

关 键 词：血管基质层细胞 脂肪来源干细胞 脂肪移植 

分 类 号：R622[医药卫生—整形外科]