Effect of Extracts from Radix Ginseng,Radix Notoginseng and Rhizoma Chuanxiong on Delaying Aging of Vascular Smooth Muscle Cells in Aged Rats  

作　　者：陶丽丽 雷燕 王国利 朱凌群 王洋 

机构地区：[1]Xiyuan Hospital,China Academy of Chinese Medical Sciences [2]Research Department,China Academy of Chinese Medical Sciences [3]Dongzhimen Hospital,Beijing University of Chinese Medicine

出　　处：《Chinese Journal of Integrative Medicine》2012年第8期582-590,共9页中国结合医学杂志（英文版）

基　　金：Supported by the National Basic Research Program of China (Program 937,No.2007CB507400);the National Natural Science Foundation of China(No.30973828);the Independent Topic Program of China Academy of Chinese Medical Sciences (No.Z02151)

摘　　要：Objective： To observe the effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong （EXT） on delaying vascular smooth muscle cells （VSMCs） aging in aged rats. Methods： VSMCs were obtained by the modified tissue explants technique and were shown to be positive for smooth muscle α-actin （SM-α-actin） by immunohistochemistry staining. VSMCs obtained from the young rats were served as the young control group; VSMCs obtained from the old rats were treated with no drug （the old group）, with low dose extracts （20 mg/L, the EXT low-concentration group） and high dose extracts （40 mg/L, the EXT highconcentration group）, and with Probucal （106 mol/L, the Probucal group） as a positive control. All groups were cultured for 24 h in the medium with 10% serum for 24 h followed by another 24 h in the serum-free medium. At the end of the 48-h culture, the following analyses were performed including determination of senescenceassociated β-galactosidase （SA β-Gal） activity, flow cytometry analysis of cell cycle, real-time quantitative reverse transcription polymerase chain reaction （RT-PCR） analyses of p16, Cyclin D1, cyclin-dependent kinase 4 （CDK4） and retinoblastoma （Rb） mRNA expression, and Western blotting analyses of p16, cyclin D1, CDK4 and phosphoretinoblastoma （pRb） protein expressions. Results： （1） In comparison to the younger rats, VSMCs from aged rats had significantly more SA β-Gal positive cells （P〈0.01） and more cells in S phase （P〈0.05）. VSMCs from the all treated groups showed a significant decrease in both SA β-Gal positive cells （P〈0.05） and S phase （P〈0.05） compared to the old rats. （2） Compared with the young group, VSMCs in the old group had a significant decrease in p16 and Rb mRNA expression and a significant increase in Cyclin D1 and CDK4 mRNA expression. Compared with the old group, VSMCs in the treated groups had a significant increase in p16 and Rb mRNA expression and a significant decrease in Objective： To observe the effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong （EXT） on delaying vascular smooth muscle cells （VSMCs） aging in aged rats. Methods： VSMCs were obtained by the modified tissue explants technique and were shown to be positive for smooth muscle α-actin （SM-α-actin） by immunohistochemistry staining. VSMCs obtained from the young rats were served as the young control group; VSMCs obtained from the old rats were treated with no drug （the old group）, with low dose extracts （20 mg/L, the EXT low-concentration group） and high dose extracts （40 mg/L, the EXT highconcentration group）, and with Probucal （106 mol/L, the Probucal group） as a positive control. All groups were cultured for 24 h in the medium with 10% serum for 24 h followed by another 24 h in the serum-free medium. At the end of the 48-h culture, the following analyses were performed including determination of senescenceassociated β-galactosidase （SA β-Gal） activity, flow cytometry analysis of cell cycle, real-time quantitative reverse transcription polymerase chain reaction （RT-PCR） analyses of p16, Cyclin D1, cyclin-dependent kinase 4 （CDK4） and retinoblastoma （Rb） mRNA expression, and Western blotting analyses of p16, cyclin D1, CDK4 and phosphoretinoblastoma （pRb） protein expressions. Results： （1） In comparison to the younger rats, VSMCs from aged rats had significantly more SA β-Gal positive cells （P〈0.01） and more cells in S phase （P〈0.05）. VSMCs from the all treated groups showed a significant decrease in both SA β-Gal positive cells （P〈0.05） and S phase （P〈0.05） compared to the old rats. （2） Compared with the young group, VSMCs in the old group had a significant decrease in p16 and Rb mRNA expression and a significant increase in Cyclin D1 and CDK4 mRNA expression. Compared with the old group, VSMCs in the treated groups had a significant increase in p16 and Rb mRNA expression and a significant decrease in

关 键 词：vascular aging vascular smooth muscle cells extracts from Radix Ginseng Radix Notoginseng ted β -galactosidase P16 

分 类 号：R285.5[医药卫生—中药学]