不同形式游泳运动对大鼠延髓腹外侧区c-fos表达的影响  

The effects on the expression of c-fos in ventrolateral medulla of rats treating with different swimming exercises

作　　者：宋慧芳[1] 李楠 杨李旺[3] 王迎春[4] 闫八一[1] 杨桂姣[1] 

机构地区：[1]山西医科大学人体解剖学教研室,太原030001 [2]太原卫校人体解剖学教研室,太原030012 [3]山西中医学院人体解剖学教研室,太原030024 [4]第四军医大学西京骨科医院,西安710032

出　　处：《神经解剖学杂志》2012年第4期380-384,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30671019);山西医科大学科技创新基金项资助目(01200803)

摘　　要：目的:探讨不同形式游泳运动后大鼠延髓腹外侧区c-fos的表达及其时效性分布规律。方法:选取正常成年雄性SD大鼠55只,随机分为对照组(5只)、持续游泳运动组(25只)和间歇游泳运动组(25只)。运动组进行6周游泳训练建立运动模型。运动结束后0、0.5、1、2、4 h等不同时间点取材,免疫组化SABC法检测延髓腹外侧区c-fos阳性神经元数目的变化。结果:(1)延髓腹外侧区头端(RVLM)持续组大鼠c-fos阳性神经元数目在运动结束后逐渐增多,至0.5 h达高峰,之后缓慢回落,至2 h后接近对照组水平。间歇组运动后即刻即可见c-fos阳性神经元数目显著增高至峰值,之后维持于较高的水平范围内呈现缓慢下降趋势,至4 h恢复至对照水平;(2)延髓腹外侧区尾端(CVLM)持续游泳运动组大鼠c-fos阳性神经元数目在运动结束后略有增加(P>0.05),0.5 h迅速下降至低于对照组水平(P<0.05),之后重新回升至对照水平,并逐渐降低。间歇游泳运动组,运动后c-fos阳性神经元数目缓慢上升,至1 h达高峰,之后回落至2 h后再次略微升高。结论:不同形式游泳运动可引起大鼠延髓腹外侧区c-fos信号通路不同程度的活化。Objective： To explore the expression of c-fos and the chronergy distribution pattern in ventrolateral medulla （VLM） of rats that treating with different kinds of swimming exercises. Methods： Fifty-five adult male SD rats were ran- domly divided into control group （ n = 5 ） , endurance swimming group （ n = 25 ） and interval swimming group （ n = 25 ）. The rats in the latter two groups were trained for 6 weeks. The brains were removed at the moments of 0, 0.5, 1,2, 4 h after swimming, the changes of the number of c-los immunopositive neurons in VLM were detected by immunohistoehemi- eal SABC techniques. Results： （ 1 ） In the endurance swimming group the number of c-fos immunopositive neurons in- creased gradually and reached to the top at 0.5 h, then reduced to the level that was equal to the control group until 2 h at rostral ventrolateral medulla （RVML）. And the number of c-fos immunopositive neurons in interval swimming group peaked at 0 h, remained at the high level and turned down slowly until 4 h to the control level at RVML; （2） In the en- durance swimming group, the number of c-los immunopositive neurons showed a nonstatistically increase （ P 〉 0.05 ） and then reduced quickly at 0.5 h, then it increased again to the control level and reduced gradually in caudal ventrolateral medulla （CVLM）. Moreover, the number of c-fos immunopositive neurons in interval swimming group peaked at 1 h, turned down until 2 h, and then increased again at CVLM. Conclusion： The different kinds of swimming exercises can induce the different activation level of c-fos signal pathway in VLM of rat.

关 键 词：游泳运动 延髓腹外侧区 C-FOS 大鼠 

分 类 号：R87[医药卫生—运动医学]