GASA基因超家族新成员——烟草抗菌肽基因的克隆及序列分析  

Cloning and sequence analysis of tobacco antimicrobial peptide genes,novel members of GASA gene superfamily

作　　者：林世锋[1] 任学良[1] 王东茂[2] 张拓[2] 王仁刚[1] 

机构地区：[1]贵州省烟草科学研究所,贵阳550081 [2]北京蛋白质组研究中心/蛋白质组学国家重点实验室,北京102206

出　　处：《中国烟草学报》2012年第3期97-102,共6页Acta Tabacaria Sinica

基　　金：贵州省烟草专卖局(公司)科技专项(200911);中国烟草总公司重点项目(2010-221);贵州省科学技术基金项目(黔科合J字[2012]2256号)

摘　　要：抗菌肽可以增强植物对病原微生物的抵抗能力,本研究利用生物信息学方法,获得了烟草品种K326的3种抗菌肽cDNA序列(NtSN1a、NtSN1b和NtSN2a),并对其进行了序列分析。结果表明,3种抗菌肽cDNA序列均具有完整的开放读码框,NtSN1a和NtSN1b均编码88个氨基酸,NtSN2a编码104个氨基酸。对推导的氨基酸序列分析发现,它们都具有典型的GASA基因超家族保守结构域,属于GASA基因超家族成员。系统进化分析表明,NtSN1a和NtSN1b与马铃薯抗菌肽snaking-1聚为一类,NtSN2a与马铃薯抗菌肽snaking-2聚为一类。这些结果为进一步研究抗菌肽基因的抑菌效应,比较其在烟草抗病防御反应中的作用奠定了基础。Antimicrobial peptides （AMPs） have been considered to play a key role in plant defense. Three distinct AMP cDNAs （NtSNla, NtSNlb and NtSN2a） were cloned from tobacco cultivar K326 using in silicon cloning technique, and were analyzed by bioinformaties method. Sequence analysis revealed that both NtSNla and NtSNlb encoded an open- reading frame of 88 amino acids, and NtSN2a encoded an open-reading frame of 104 amino acids. , all of them shared the conserved domain of the GASA superfamily. Phylogenetie analysis showed that NtSNla and NtSNlb cluster with po-tato snaking-1, and NtSN2a clusters with potato snaking-2. These results lay the foundation for clarifying the inhibitory effect of AMPs, discovering their functional differences and understanding the molecular mechanisms underlying tobacco defense responses.

关 键 词：烟草 GASA基因超家族 抗菌肽 克隆 序列分析 

分 类 号：S572[农业科学—烟草工业]