肿瘤坏死因子-α对人表皮黑素细胞增殖及黑素合成的影响研究  

Study on Effects of TNF-α on Human Epidermis Melanin Cell Proliferation and Melanin Synthesis

作　　者：林新瑜[1] 王芳[1] 林鸿刚[2] 段西凌[1] 

机构地区：[1]四川省医学科学院.四川省人民医院皮肤病性病研究所,四川成都610072 [2]四川大学国家生物治疗重点实验室,四川成都610041

出　　处：《成都医学院学报》2012年第2期187-189,共3页Journal of Chengdu Medical College

基　　金：四川省卫生厅基金资助(NO:110219)

摘　　要：目的建立正常人表皮黑素细胞体外培养体系,观察不同浓度肿瘤坏死因子-α对体外培养正常人黑素细胞的细胞增殖及黑素合成的影响。方法采用四甲基偶氮唑盐(MTT)法测定不同浓度肿瘤坏死因子-α对黑素细胞增值的影响,NaOH裂解法测定黑素生成量,流式细胞仪检测黑素细胞的凋亡率。结果肿瘤坏死因子-α对黑素细胞活力有抑制作用,能使细胞增殖能力降低,黑素合成减少,增加黑素细胞的凋亡率。结论肿瘤坏死因子-α对体外培养的黑素细胞增殖及黑素生成都有抑制作用,这为临床应用肿瘤坏死因子-α治疗色素性疾病提供了实验依据。Objective Establishing the cultured system of human normal melanocytes in vitro, and studying effects of TNF-α on cultured human melanocytes. Methods MTT method,NaOH assay were employed to measure the rate of proliferation,melanin synthesis of melanocytes respectively;apoptosis rates of meianocytes were detected by using flow cytometer. Results TNF-α showed a significant decrease of the rate of melanocyte proliferation in concentration and time dependent manner,inhibited melanin synthesis and increased melanocyte apoptosis. Conclusion It suggests that TNF-α at the concentration of 50 200 ng/ml is able to suppress melanocytic proliferation and melanin synthesis. It may be developed to be a potential skin lightening agent and worth to study later.

关 键 词：肿瘤坏死因子Α 黑素细胞 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]