骨关节炎软骨细胞凋亡调控基因的研究  

Control genes of chondrocyte apoptosis in osteoarthritic articular cartilage

作　　者：胡建华[1] 黄公怡[1] 黄尚志[2] 杨丽[3] 

机构地区：[1]北京医院骨科,100730 [2]中国医学科学院基础研究所遗传室 [3]北京医院,100730

出　　处：《中华外科杂志》2000年第4期266-268,I017,共4页Chinese Journal of Surgery

摘　　要：目的　比较分析正常人及老年性骨关节炎患者软骨细胞bax和bcl 2的表达及细胞凋亡状况。　方法　取 9例骨关节炎患者的关节软骨做实验标本 ,以 6例无骨关节炎病史的意外死亡者关节软骨作为正常对照 ;采用逆转录 /聚合酶链反应 (RT PCR)方法检测bax和bcl 2mRNA表达 ,免疫组化检测bax和bcl 2蛋白 ;应用TUNEL方法进行凋亡细胞原位检测。　结果　骨关节炎患者和正常对照软骨细胞都能表达bax和bcl 2mRNA ;骨关节炎关节软骨细胞baxmRNA表达量较正常对照显著增高 (P <0 0 1) ,bcl 2mRNA表达量也高于正常对照组 (P <0 0 5 ) ,两组间bax/bcl 2表达量的比值差异无显著性意义 (P >0 0 5 ) ;免疫组化可检测到相应表达水平的蛋白 ;骨关节炎软骨细胞凋亡 (4%～ 14% )多于正常对照 (0～ 2 % )。　结论　软骨细胞凋亡受bax和bcl 2共同调节 ;bax和bcl 2的共同调节结果可能是OA患者软骨细胞凋亡增加 ,但凋亡率不高。Objective To investigate the expression of bax and bcl 2 in normal human articular chondrocytes and in osteoarthritic articular cartilage. MethodsThe samples of articular cartilage were obtained from 9 patients and 6 normal subjects. Bax and bcl 2 mRNA were detected by reverse transcriptase/polymerase chain reaction (RT PCR) and their expression proteins were analyzed immunohistochemiscally. TUNEL technique was used to study apoptosis in situ.]Results Bax and bcl 2 mRNA was detectable in chondrocytes of both osteoarthritic and normal cartilage. Bax mRNA of chondrocytes from patients with osteoarthritis(OA) was overexpressed compared with the normal controls( P <0.01), and OA cartilage chondrocyte also expressed more bcl 2 mRNA than the controls ( P <0 05). There was no statistically significant difference of bax/bcl 2 between the two groups. Immunohistochemical staining demonstrated the same level of bax and bcl 2 proteins as their mRNA. A greater proportion of apoptotic chondrocytes were found in the OA cartilage than that in normal controls (4% 14% versus 0 2%). Conclusions Our results suggested that chondrocyte apoptosis was co regulated by both bax and bcl 2. The ratio of bax to bcl 2 may contribute not only to the lower percentage of apoptotic chondrocytes, but also to the chronic pathologic process in OA.

关 键 词：软骨细胞 骨关节炎 基因调节 细胞凋亡 

分 类 号：R684.3[医药卫生—骨科学]