脂联素通过LKB1途径激活腺苷酸活化蛋白激酶  

Adiponectin activates AMP-activated protein kinase via LKB1 pathway

作　　者：邓大同[1] 王佑民[1] 程媛[1] 丁晓洁[1] 

机构地区：[1]安徽医科大学内分泌代谢病研究所,安徽医科大学第一附属医院内分泌科,安徽内分泌代谢病省级实验室 ,合肥230032

出　　处：《中华内分泌代谢杂志》2012年第7期578-583,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：安徽省教育厅自然科学重点项目（KJ2011A161）;安徽省科技攻关项目（1206c080534）

摘　　要：目的探讨脂联素是否通过LKB1途径激活骨骼肌及肝脏中腺苷酸活化蛋白激酶（AMPK）。方法将28只6周龄雄性Sprague—Dawley大鼠分为普通饮食组（Nc组，n=15）和高脂饮食组（HF组，／2=13）。喂养16周后，取空腹静脉血测定血清游离脂肪酸（FFA）、甘油三酯（TG）、总胆固醇（TC）、空腹血糖（FPG）、空腹胰岛素（FINS）及脂联素。采用Western印迹法测定各组大鼠骨骼肌及肝脏组织中AMPKα、磷酸化的AMPKcx和LKB1蛋白的表达。将原代培养的骨骼肌细胞及肝细胞分别予以脂联素和根赤壳菌素干预，免疫荧光技术测定各组细胞中AMPKα、磷酸化AMPKα和LKB1蛋白的表达。结果与NC组比较，HF组大鼠体重、FFA、TG、FPG、FINS均升高（均P〈0．05），脂联素水平降低（P〈0．05），骨骼肌及肝组织中AMPKoL磷酸化和LKB1蛋白表达水平降低（均P〈0．05）。原代培养大鼠骨骼肌细胞及肝细胞中脂联素显著增加AMPKα磷酸化及LKB1表达水平（均P〈0．05），加入根赤壳菌素表达明显降低（均P〈0．05）。结论脂联素在大鼠骨骼肌和肝脏组织可能通过LKB1途径激活AMPK。Objective To explore whether adiponectin activates AMP-activated protein kinase （AMPK） via LKB1 pathway or not in skeletal muscle and liver tissues. Methods Male Sprague-Dawley rats （ n = 28） were divided into normal control diet （ N C, n = 15 ） and high-fat diet （ HF, n = 13 ） groups. After 16 weeks feeding, fasting blood free fatty acids （ FFA ）, triglyceride （ TG）, total cholesterol （ TC ）, fasting plasma glucose （ FPG）, fasting insulin （ FINS ）, and adiponectin were determined. The protein levels of AMPKα, phosphorylated AMPKα （p-AMPK） , and LKB1 in the skeletal muscle and liver tissues were analyzed with Western blot. Cultured primary skeletal muscle cells and hepatic cells were incubated with adiponectin and radicicol. The expression of AMPKα, p-AMPKα, and LKB1 in these cells were analyzed with immunofluorescence method. Results Compared with NC group, body weight, FFA, TG, FPG, and FINS in rats of HF group were significantly higher （ all P〈0.05 ） while serum adiponectin level was lower（ P〈0. 05 ）. The levels of AMPKα phospholTlation and LKBI expression in the skeletal muscle and liver tissues of HF group were lower than those in NC group. In primary skeletal muscle cells and hepatic cells, adiponectin significantly increased the levels of AMPKα phosphorylation and LKB1 expression （ all P 〈 0. 05 ） , which were decreased by radicicol（P〈0. 05）. Conclusion Adiponectin may activate AMPK via LKBI pathway in skeletal muscle and liver tissues of rats.

关 键 词：脂联素:LKB1 腺苷酸活化蛋白激酶 

分 类 号：R285.5[医药卫生—中药学]