马先蒿无性系建立的研究  

Establishment of Clone for Pedicularis

作　　者：李冬雪[1] 高珂[1] 宋诗迎[1] 徐娜[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《绿色科技》2012年第7期53-55,共3页Journal of Green Science and Technology

基　　金：辽宁省高等教育教学改革项目(编号:20090304);辽宁师范大学教学改革项目(编号:LSJG20090108)资助

摘　　要：为满足马先蒿观赏栽培对种苗的需要,以生长点为材料,采用组织培养的方法,进行了愈伤组织诱导与分化、试管苗的生根与生根继代增殖培养,以及移栽和定植的研究。结果表明:MS+6-BA0.6mg·L-1+2,4-D1.0mg·L-1+NAA0.3mg·L-1是生长点愈伤组织诱导和分化培养的理想培养基;1/3MS+ABT2号10mg·L-1是不定芽生根培养和试管苗生根继代增殖培养的理想培养基;试管苗移栽和定植的成活率分别为93.4%、98.5%,定植的试管苗保持了马先蒿的植物学性状和观赏性状。In order to meet the need of germchits for cultivation,the growing points of Pedicularis are used as materials to do the study by using tissue culture methods. The study is mainly about callus induction and dif- ferentiation,rooting and subculturing of tube seedlings, and tube seedlings transpJanting colonization. The results demonstrate that the ideal medium for callus induction and differentiation is MS＋6--BA0.6mg·L-1 ~2,4--D1.0mg·L-1＋NAA0.3mg·L-1,and 1/3MS＋ABTH10mg·L-1 is the optimum medium for roo- ting and subculturing;the survival rate of transplanting and stable planting is 93.4% and 98.5%respective- ly. The field planting plantlets maintain all botanical traits and ornamental traits of wild Pedieularis.

关 键 词：马先蒿 组织培养 无性系 

分 类 号：O657.39[理学—分析化学]