黄体生成素干预对体外培养小鼠卵巢VEGF和Survivin表达的影响  

Effects of LH Intervention on the Expressions of Cultured Mouse Ovarian VEGF and Survivin

作　　者：张晓光[1,2] 张庭元[1,2] 杨旭[1,2] 陈杰[1,2] 王燕蓉[1,2] 裴秀英[1,3] 

机构地区：[1]宁夏医科大学生育力保持省部共建教育部重点实验室宁夏生殖与遗传重点实验室,银川750004 [2]宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川750004 [3]宁夏医科大学基础医学院生物化学与分子生物学系,银川750004

出　　处：《宁夏医科大学学报》2012年第7期663-665,672,F0002,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81160085);宁夏自然科学基金重点项目(NZ1084);宁夏科技攻关项目(2007GG071)

摘　　要：目的探索黄体生成素(LH)对小鼠卵巢体外培养后血管内皮生长因子(VEGF)和存活素(Survivin)表达的影响。方法将从8周龄小鼠新鲜取材的半卵巢分别在无LH(培养对照组)和添加LH(0.15IU.mL-1)干预下培养3、6、12、24h后通过免疫组化法观察半卵巢VEGF、Survivin的表达。结果培养组VEGF、Survivin表达比新鲜对照组降低且呈逐渐降低趋势;在培养3、6、12h阶段,LH 0.15干预后VEGF阳性表达高于培养对照组(P<0.05);3h和12h Survivin的表达均高于培养对照组(P<0.05)。结论 LH体外干预可上调小鼠卵巢的VEGF、Survivin表达,VEGF和Survivin表达呈正相关。Objective To explore the effects of the intervention of luteinizing hormone（LH） on the expression of ovarian angiogenic factors of VEGF and Survivin in cultured mouse ovarians in vitro.Methods The expression of VEGF and Survivin were monitored by immunohistochemical method after the hemi-ovaries of juvenescent mice had been cultured in the culturing mediums that contain LH of 0.00IU.mL-1 and 0.15IU.mL-1 for 3,6,12,and 24hours.Results The expression of VEGF and Survivin in culture group was significantly lower than that in fresh control group.The expression of VEGF in LH group was higher than that in non-LH group at 3h,6h,12h（P 〈 0.05）.The expression of Survivin in LH group was higher than that in non-LH group at 3h,12h.（P 〈 0.05）.Conclusion LH intervention in vitro can increase expression of VEGF and Survivin in cultured mouse ovarians in vitro.

关 键 词：黄体生成素 存活素 血管内皮生长因子 小鼠卵巢 体外培养 

分 类 号：R711.75[医药卫生—妇产科学]