牙釉质基因鉴定麇鹿性别  

Sex Determination of Pere David's Deer(Elaphurus davidianus) Based on DNA Analysis of the Amelogenin Gene

作　　者：武会娟[1] 张林源[2] 王文[1] 孟浩 李凯 高庆华 

机构地区：[1]北京市理化分析测试中心,北京100089 [2]北京麋鹿生态实验中心,北京100076 [3]新疆兵团畜牧科技重点实验室,阿拉尔843300

出　　处：《野生动物》2012年第4期177-179,195,共4页

摘　　要：性别鉴定是调查野生种群雌雄性比的重要方法,对野生动物种群管理具有重要意义。而牙釉质(AMEL)基因在性染色体上具有较高的保守性,在鉴定动物性别方面得到了应用,本次试验采用北京麋鹿生态实验中心的15头糜鹿组织样本,其中11个来自雄性麇鹿鹿茸,4个来自雌性糜鹿静脉血液。对所取样本分别进行基因组DNA提取、AMEL基因片段PCR扩增、纯化、测序。得到了AMEL基因鉴定和实际雌雄性别个数差异不显著(P>0.05)。结果表明:雌性麋鹿产生1条322 bpX带和1条N带,雄性麋鹿则产生322 bpX带和277 bpY带以及1条N带,雄鹿(10/10)和雌鹿(4/4)性别鉴定结果分别都与实际性别符合,所以,使用鹿茸角和血液样本进行AMEL.基因扩增电泳分析可以对糜鹿性别鉴定。Sex determination is an important tool for study and management of wildlife populations.The enamel（AMEL）gene in the sex chromosomes is highly conservative and has been used in sex identification of animals.Tissue samples from 15 Pere David’s deers at Beijing Milu Ecological Research Center were used in this study,of which eleven samples were from male deer antlers and four samples were female venous blood.DNA was extracted from the fifteen samples,AMEL gene fragments were amplified by polymerase chain reaction（PCR）.PCR products were purified and then sequenced.There was no significant difference between the results of AMEL gene sex identification and the actual gender（P〉0.05）.PCR products of female deer were an X band（322 bp）and an N band,while those of male deer were an X band（322 bp）,a Y band（277 bp） and an N band.The sex determination results of male deer（10/10）and female deer（4/4）matched the actual sex.So the sex of Pere David’s deer can be determined by electrophoresis analysis of PCR products of partial AMEL gene using DNA extracted from antlers and blood samples.

关 键 词：麋鹿 性别鉴定 牙釉质基因 PCR 

分 类 号：Q344.2[生物学—遗传学]