水稻抗纹枯病QTLqSB-9TQ和抗条纹叶枯病基因Stv-bi的聚合育种  

Rice Pyramiding Breeding Using Sheath Blight Resistance QTL qSB-9^(TQ) and Stripe Disease Resistance Gene Stv-b^i

作　　者：陈宗祥[1] 左示敏[1] 张亚芳[1] 朱俊凯[1] 王龙平[1] 冯凡[1] 马玉银[2] 潘学彪[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,植物功能基因组学教育部重点实验室,江苏扬州225009 [2]扬州职业大学,江苏扬州225000

出　　处：《作物学报》2012年第7期1178-1186,共9页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10Z165,2006AA10A103);国家转基因生物新品种培育科技重大专项(2009ZX08001-014B);国家自然科学基金项目(30900883);江苏高校作物优势学科建设工程项目资助

摘　　要：以携带抗纹枯病QTLqSB-9TQ的籼稻品种特青和携带抗条纹叶枯病基因Stv-bi的粳稻品种镇稻88为优良等位基因供体亲本,江苏省推广的粳稻品种武育粳3号和武粳15为受体亲本,分别杂交并连续回交。在回交及自交分离世代,利用开发的覆盖目标基因区间的双侧分子标记对目标基因进行辅助选择。至回交BC4F1世代,同一遗传背景2个回交方向的中选单株间聚合杂交,获得2个目标基因位点均纯合的聚合F3株系。条纹叶枯病抗性鉴定和纹枯病抗性接种鉴定结果表明,聚合株系对条纹叶枯病均表现抗病;以0～9级评级标准评价,聚合株系的纹枯病较相应的轮回亲本分别低1.1～1.6级和0.8～1.4级。结合回交低世代抗性鉴定结果分析,自行开发的分子标记对目标基因的辅助选择是有效的。讨论了抗纹枯病育种及分子标记辅助选择聚合育种的相关问题。Indica rice cultivar Teqing and japonica rice cultivar Zhendao 88 were used as the donor parents with the sheath blight （SB） resistance QTL qSB-9TQ and the resistance gene Stv-bi, respectively. Commercial rice japonica cultivars, Wuyujing 3 and Wujing 15, were used as the receipt parents to cross and backcross with the donor parents, respectively. Flanked polymorphic markers to the two loci were developed and employed to select the target loci via marker assisted selection （MAS） in each back- cross and self-cross progeny. Till backcross generation BC4F1, pyramiding was performed by crossing the selected individuals with different integression genes. At F3 generation, the pyramiding lines with homozygosity at both gene loci were obtained. Re- sults of disease resistance evaluation indicated that both pyramiding lines were resistant to stripe disease, and increased resistance to SB with a reducing SB sating of 1.1-1.6 in Wuyujing 3 background and 0.8-1.4 in Wujing 15 background based on a ＂0-9＂ SB rating scale. Combining the results of resistance phenotype at the low backcrossing generation, we thought the markers developed in this study were efficient in selecting the two genes by MAS. Some questions associated with SB resistance breeding and pyra- miding breeding via MAS were discussed.

关 键 词：分子标记辅助选择 纹枯病 条纹叶枯病 聚合育种 

分 类 号：S511[农业科学—作物学]