大豆种质资源叶型和荚粒性状的关系及与SSR标记的关联分析  

Relationship of Leaflet Shape,Pod Traits and Association with SSR Markers in Soybean Germplasm

作　　者：伍宝朵[1] 陈海峰[1] 郭丹丹[1] 沙爱华[1] 单志慧[1] 张晓娟[1] 杨中路[1] 邱德珍[1] 陈水莲[1] 朱晓玲[1] 张婵娟[1] 周蓉[1] 周新安[1] 

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学重点开放实验室,湖北武汉430062

出　　处：《作物学报》2012年第7期1196-1204,共9页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30871554;30900906);中国农业科学院公益性院所科研基金(1610172011006);作物种质资源保护项目(NB08-2130315-06);国家转基因生物新品种培育重大专项(2008ZX08004-005;2009ZX08009-133B;2009ZX08009-120B)资助

摘　　要：选用167份国内外大豆品种资源材料,依据叶长/宽比值将其划分为宽叶型和窄叶型,分析叶型相关性状与荚粒性状的相关性,结果表明叶宽、叶长/宽比均与每荚粒数显著相关(r=-0.69和0.64,P<0.001)。选用均匀分布于大豆20条连锁群上的65个SSR标记分析资源材料的群体结构,并用目标区间内13个SSR标记与叶型相关性状和荚粒性状进行关联分析,结果显示,标记20-285与每荚粒数显著关联,推测控制每荚粒数的基因可能位于标记20-285附近;标记20-26和20-45间的标记几乎都与叶长/宽比存在显著的关联性,推测控制叶型的基因位于标记20-26和20-45之间。相关分析和关联分析证实大豆叶型与荚粒性状存在密切关系,并缩小了每荚粒数和叶型候选基因的区间。Pod traits are important factors affecting soybean yield, and they have been identified to tightly associate with leaflet shape. In present study, leaflet shape/pod traits were analyzed using 167 soybean accessions, which were classified into two types （broad and narrow leaflets） based on the ratio of leaf length/width. Correlation analysis was carded out between leaflet shape re- lated traits and pod traits; ANOVA analysis was also performed using 65 SSR markers, evenly distributed in 20 linkage groups; association analysis was carried out using 13 SSR markers on the target regions. Marker 20-285, locating on chromosome I, highly associated with number of seeds per pod, indicating that the gene controlling number of seeds per pod may co-locate with marker 20-285. Almost all markers between 20-26 and 20-45 were associated with ratio of leaf length/width. We speculated that the genes controlling leaflet shape were located in the region from marker 20-26 to 20-45. Hence, it was proved the target region harboring candidate genes of leaflet shape and pod number was narrowed. Our study may provide a potential approach in molecu- lar marker-assisted breeding of seeds per pod, and a theoretical guide for cloning the gene of seeds per pod.

关 键 词：大豆 叶型 荚粒性状 相关性分析 关联分析 

分 类 号：S565.1[农业科学—作物学]