HPLC法测定白木香叶和远志地上部分芒果苷的含量  

HPLC determination of mangiferin in the leaves of Aquilaria sinensis and the different aerial parts of Polygala tenuifolia

作　　者：杨晓娟[1,2] 邹萍萍[1,2] 周观燊[1,2] 屠鹏飞[1,2] 姜勇[1,2] 

机构地区：[1]北京大学药学院天然药物与仿生药物国家重点实验室,北京100191 [2]北京大学中医药现代研究中心,北京100191

出　　处：《药物分析杂志》2012年第7期1175-1178,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：教育部新世纪优秀人才计划(985-2-102-113);"重大新药创制专项"(2009ZX09311-004;2009ZX09308-004)

摘　　要：目的:建立测定白木香叶和远志地上各部分(包括远志整个地上部分、远志叶和远志茎)芒果苷含量的高效液相色谱法,为寻找富含芒果苷的植物资源奠定基础。方法:采用Kromasil 100C18(4.6 mm"250 mm,5μm)色谱柱,以乙腈-0.5%磷酸溶液(15∶85)为流动相,等度洗脱10 min,流速为1 mL.min-1,检测波长为258 nm,柱温为30℃。结果:在本色谱条件下,芒果苷和样品中其他成分分离良好,并且浓度在0.116～580μg.mL-1范围内线性良好。加样回收率(n=6)分别为97.5%(白木香叶),97.3%(远志地上部分),97.1%(远志叶),98.5%(远志茎);RSD分别为1.6%,2.1%,1.8%,0.21%。结论:本方法操作简单、快捷、准确,重复性好,可用于测定白木香叶、远志地上部分等不同来源植物中芒果苷的含量。Objective： To develop an HPLC method to determine the content of mangiferin in the leaves of Aquilar- ia sinensis and in the different aerial parts of Polygala tenuifolia （ including the total aerial parts of P. tenuifolia, the leaves of P. tenuifolia and the stems of P. tenuifolia）, to acquire some insight for mangiferin - rich herbs. Methods： Chromatographic separation was carried out on a Kromasil 100 A C18 （4. 6 mm × 250 mm, 5μm） col- umn with an isocratic elution for 10 min at a flow rate of 1.0 mL .min^-1 The mobile phase was composed of ace- tonitrile and 0. 5% H3PO4 aqueous solution（15：85）. The detection wavelength was set at 258 nm, and the column temperature was 30 ℃. Results：Mangiferin was separated well with other components in these herbs with a linear range of 0. 116 -580 μg . mL^-1. The mean recoveries were 97.5% （the leaves ofA. sinensis） , 97.3% （the aerial parts of P. tenuifolia）, 97. 1% （the leaves of P. tenuifolia）, 98.5% （the stems of P. tenuifolia）, and RSDs were 1.6%, 2. 1%, 1.8% and 0. 21%, respectively. Conclusion：This method is simple, rapid, repeatable and accurate, and can be used for the quantitation of mangiferin in these herbs.

关 键 词：芒果苷(芒果素 知母宁) 多羟基酚类化合物 酮碳苷 白木香叶 远志地上部分 叶 茎 含量测定 高效液相色谱 中药综合开发利用 

分 类 号：R917[医药卫生—药物分析学]