催化荧光法测定富硒中药中的硒(Ⅳ)含量  

Determination of selenium content in abundant selenium herbal drugs by catalytic fluorescence analysis method

作　　者：刘卫洁[1] 杨贵亭[1] 王胜利[1] 李晓利[1] 

机构地区：[1]邯郸学院化学系,邯郸056001

出　　处：《药物分析杂志》2012年第7期1179-1182,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立富硒中药材中硒的测定方法。方法:在稀硫酸介质中,痕量硒(Ⅳ)能够催化溴酸钾氧化中性红使其发生荧光猝灭,且催化前后中性红荧光强度的变化(ΔF)与硒的含量在一定范围内呈良好的线性关系,摸索了实验条件对催化体系的影响。结果:方法的线性范围为12～60μg.L-1,检出限为5.2μg.L-1,回收率为95%～105%。结论:该方法简便、快速、准确,可作为中草药中痕量硒的一种快速分析方法。Objective ： To establish a new method based on fluorescence quenching for the determination of trace se- lenium （Se） in abundant selenium herbal drugs. Method： Trace Se （ Ⅳ ） could catalyze the oxidation of neutral red by potassium bromate under the condition of fluorescence quenching in the medium of dilute sulfuric acid. The values （△F）of fluorescence intensity before and after the catalyzation kept a good liner relationship with the concen- tration of Se （ Ⅳ ） within a certain range. The effect of the experimental conditions on catalyzation system was also studied. Result：The linear range of the method was 12 -60 μg . L^-1 , and the detection limit was 5.2μg . L^-1. The range of recovery rate was 95% -105%. Conclusion： The method is simple, fast and accurate. As a new an- alytical method, it can be used to determinate trace selenium in herbal drugs rapidly.

关 键 词：溴酸钾 中性红 催化荧光法 硒(Ⅳ) 黄芪 党参 鸭胆子 微量元素测定 

分 类 号：R917[医药卫生—药物分析学]