RP-HPLC法测定苦木药材中4-甲氧基-5-羟基铁屎米酮的含量  

RP-HPLC determination of 4-methoxy-5-hydroxycanthin-6-one in herbal medicines Picrasma quassioides(D.Don) Benn

作　　者：赵文娜[1] 余静洁[1] 张新新[1] 陈琳[1] 孙文基[1] 

机构地区：[1]西北大学陕西省生物医药重点实验室,西安710069

出　　处：《药物分析杂志》2012年第7期1239-1242,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：西北大学研究生创新基金项目(10YZZ34)

摘　　要：目的:建立RP-HPLC法测定苦木药材中4-甲氧基-5-羟基铁屎米酮含量。方法:采用Welchrom C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%醋酸水溶液(30∶70)为流动相,流速1.0 mL.min-1,紫外检测器检测,检测波长254 nm,柱温30℃。结果:4-甲氧基-5-羟基铁屎米酮浓度在12.0～240.0μg.mL-1(r=0.9999)范围内与峰面积呈良好的线性关系,平均回收率(n=6)为101.2%,RSD为2.3%。结论:该方法简便、准确、灵敏,为2015年版中国药典一部增订苦木含量测定项提供参考。Objective： To develop an HPLC method for the determination content of 4 - methoxy - 5 - hydroxycan- thin -6 -one in Picrasma quassioides （D. Don） Benn.. Methods： Welchrom C18 （250 mm ×4.6 mm, 5 μm） column was adopted and the mobile phase was mixture of acetonitrile and 0. 1% acetic acid（30： 70） with the flow rate of 1.0 mL . min^- 1 ; The detection wavelength was 254 nm, and the column temperature was 30 ℃. Results： The calibration curve was linear in the range of 12.0 - 240.0 μg . mL-1 for 4 - methoxy - 5 - hydroxycanthin - 6- one（r =0. 9999）, and the average recovery was 101.2% with RSD of 2. 3%. Conclusions： This method is simple, accurate, sensitive, which can offer references for the quality control of herbal medicines Picrasma quas- sioides （ D. Don） Benn. in China Pharmacopoeia（2015 Edition）.

关 键 词：反相高效液相色谱法 苦木 4-甲氧基-5-羟基铁屎米酮 含量测定 质量控制 标准完善 

分 类 号：R917[医药卫生—药物分析学]