续断高效毛细管电泳指纹图谱研究  

Study on high performance capillary electrophoresis fingerprint of Dipsaci Radix

作　　者：吴浩[1] 顾晓风[1] 辛妮[1] 杨中林[2] 胡育筑[1] 

机构地区：[1]中国药科大学分析化学教研室,药物质量与安全预警教育部重点实验室,南京210009 [2]中国药科大学现代中药重点实验室,南京210009

出　　处：《药物分析杂志》2012年第7期1259-1263,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金重点项目(No.30730113)资助

摘　　要：目的:利用高效毛细管电泳(HPCE)方法建立中药续断的指纹图谱。方法:采用50μm×65 cm(有效长度57 cm)未涂层石英毛细管柱,以pH 9.20,50 mmol.L-1的硼砂-硼酸为缓冲溶液体系,运行电压25 kV,温度25℃,压力进样5 kPa×30 s,检测波长212 nm。采用"中药色谱指纹图谱相似度评价系统2004A版"软件和聚类分析方法,对样品图谱进行分析。结果:12批续断HPCE指纹图谱共确认了14个共有指纹峰,其中产自湖北的2批药材相似度介于0.8至0.9之间,产自四川的10批药材相似度大于0.9。聚类与相似度结果一致。结论:此法准确、可靠,可为续断药材的质量评价与控制提供科学依据。Objective： To investigate the HPCE fingerprint of Dipsaci Radix. Methods： The HPCE fingerprint was es- tablished using a 50μm× 65 cm uncoated fused silica capillary（effective length 57 cm） ,a buffer comprising 50 mmol . L^- 1 borate -boric acid at pH 9.20, a running voltage of 25 kV, a capillary temperature of 25 ℃ and a wavelength of 212 nm. The sample was injected under pressure of 5 kPa for 30 s. The HPCE fingerprint was evaluated by the software ＂Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine Version 2004A＂ and clustering analysis. Results： By analyzing the HPCE fingerprint, high similarities were found among the 12 samples. The similarity coefficients of the two batches of samples from Hubei were from 0.8 to 0.9, and those of the remai- ning samples from Sichuan were greater than 0.9. Fourteen major co - possessing peaks were selected as the charac- teristic fingerprint peaks of Dipsaci Radix. Clustering results were consistent with the similarity analysis. Conclu- sion： The developed method is accurate and reliable, and the fingerprint analysis can thus be used for the quality assessment and control of Dipsaci Radix.

关 键 词：续断 高效毛细管电泳 指纹图谱 聚类分析 质量评价与控制 

分 类 号：R917[医药卫生—药物分析学]