大鼠空间学习记忆和海马长时程增强的相关性研究  

The correlation study between spatial memory and hippocampal long term potentiation in rats

作　　者：韩维娜[1] 原丽[1] 刘晓杰[1] 周丽薇[1] 武美娜[1] 祁金顺[1] 

机构地区：[1]山西医科大学生理学系,太原030001

出　　处：《中华行为医学与脑科学杂志》2012年第7期630-633,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：教育部高等学校博士学科点专项科研基金项目（博导类）（20101417110001）;山西省归国留学人员基金项目（2010-51）;山西省自然科学基金项目（2010011049-3）;太原市科技项目基金项目（110148041）

摘　　要：目的探讨生理或病理状态下大鼠海马突触可塑性和空间学习记忆的相关性，为在体电生理实验和行为学实验结果的一致性提供依据。方法按照随机数字表随机选择SD大鼠38只，进行Morris水迷宫空间学习记忆行为学和在体海马晚期长时程增强（L—LTP）电生理实验。主要指标包括大鼠连续5d定位航行试验寻找水下平台的逃避潜伏期、撤除平台后空间探索试验的目标象限游泳时间百分比以及高频刺激后3h的场兴奋性突触后电位（fEPSP）。结果双侧海马注射4nmolAp25-35，明显伤害了大鼠水迷宫实验中的空间学习记忆能力（P〈0．05）和在体海马L-LTP（P〈0．05）。正常对照组大鼠L-LTP与逃避潜伏期呈现明显负相关（r=-0．8306，P〈0．01），与目标象限游泳时间百分比呈现明显正相关（r=0．7709，P〈0．01）；AD模型组大鼠3h后fEPSP幅度也与逃避潜伏期具有明显负相关（r=-0．7675，P〈0．01），与目标象限游泳时间百分比正相关（r=0．8049，P〈0．01）；将两组大鼠数据合并后，海马L-LTP与逃避潜伏期和目标象限游泳时间百分比的相关性更高，分别为r=-0．9124（P〈0．01）和r=0．9745（P〈0．01）。结论大鼠在体海马L—LTP电生理记录与空间学习记忆行为学测试结果具有良好的相关性，提示海马L—LTP可能是行为学改变的电生理机制，其损伤可以在一定程度上反映动物学习记忆功能障碍。Objective To investigate the correlation of hippocampal synaptic plasticity with spatial learn- ing and memory under normal and pathological condition, and provide experimental evidence for the coincidence of hippocampal late-phase long-term potentiation （L-LTP） and behavioral experiments. Methods 38 SD rats were randomly divided into two groups, control and AD model. First, Morris water maze was used to test the ability of spatial learning and memory of rats. The escape latencies for rats to search for an underwater platform in 5 days of navigation tests and the swimming time percentage in target quadrant on the 6th day after withdrawing the platform in probe trails were recorded. Then,in vivo hippocampus L-LTP of field excitatory postsynaptic potential （fEPSP） in CA1 region was recorded after delivering high frequency stimulation （HFS）. Results Bilateral intrahippocam- pal injection of 4 nmol amyloid β peptide （ Aβ 25-35 ） significantly impaired spatial learning and memory of rats in water nmze tests, as well as in vivo hippocampal L-LTP. In control group, there was a significant negative correla- tion between the amplitude of fEPSP and the escape latency （ r = - 0. 8306, P 〈 0.01 ） and a significant positive correlation between the amplitude of tEPSP and the swimming time percentage in target quadrant （ r= 0. 7709, P〈 0.01 ）. In AD model group,similar correlations were found,with a correlation coefficient of r = -0. 7675 （P〈 0.01 ） and r=0. 8049 （P〈0.01） ,respectively. When putting all data from the two groups together,the hippocam- pal L-LTP was more correlated with escape latency （ r = -0. 9124, P 〈 0.01 ） and swimming time percentage （ r = 0. 9745, P 〈 0.01 ）. Conclusion There is very close correlation between the hippocampal L-LTP and the spa- tial learning and memory behavior in rats, suggesting that the hippocampal L-LTP may be involved in the electro- physiological mechanism of spatial learning and memory in rats, and the impairment of L-LTP could pa

关 键 词：空间学习记忆 MORRIS水迷宫 晚期长时程增强 淀粉样Β蛋白 

分 类 号：R285.5[医药卫生—中药学]