慢性乙型肝炎患者外周血IL-9和IL-10及TGF-β1检测及其意义  

IL-9,IL-10 and TGF-β1 levels in the peripheral blood of patients with chronic hepatitis B

作　　者：邓勇[1] 苏智军[1] 余雪平[1] 郭如意[1] 明德松[1] 林振忠[1] 

机构地区：[1]福建医科大学附属泉州第一医院感染科,福建泉州362000

出　　处：《中国病毒病杂志》2012年第4期296-300,共5页Chinese Journal of Viral Diseases

基　　金：泉州市优秀人才培养专项经费资助项目(10A07);泉州市科技计划项目(2011Z63)

摘　　要：目的检测慢性乙型肝炎(CHB)患者外周血细胞因子白细胞介素(IL)-9、IL-10及转化生长因子-β1(TGF-β1)的水平,并探讨其临床意义。方法采用酶联免疫吸附试验(ELISA)检测98例CHB患者和20例健康体检者(对照组)外周血中细胞因子IL-9、IL-10及TGF-β1水平。结果 CHB轻中度组、CHB重度组及肝硬化组血清IL-9水平分别为(319.96±367.26)ng/L、(308.47±402.77)ng/L、(432.49±591.65)ng/L,IL-10水平分别为(95.73±107.93)ng/L、(69.52±66.49)ng/L、(111.41±113.41)ng/L,TGF-β1水平分别为(42.40±56.57)μg/L、(138.85±178.55)μg/L、(135.32±179.44)μg/L,均明显高于正常对照组[(139.52±193.44)ng/L、(29.73±21.27)ng/L、(15.30±14.51)μg/L,P均<0.05];血清IL-9、IL-10水平与不同HBV DNA载量有关,而IL-9、IL-10及TGF-β1水平与不同的HBeAg状态无关。CHB各组IL-9与IL-10呈强正相关(r=0.932,P<0.001),IL-9与TGF-β1呈弱正相关(r=0.227,P=0.014);IL-10与TGF-β1呈弱正相关(r=0.217,P=0.018)。结论 IL-9、IL-10及TGF-β1均参与CHB的发病过程,IL-9及IL-10可能具有抑制炎症作用,其持续升高可能与CHB患者慢性感染状态有关。Objective To measure the levels of cytokines IL-9,IL-10 and TGF-β1 in the peripheral blood of patients with chronic hepatitis B（CHB）.Methods Blood samples from 98 CHB patients and 20 healthy controls were collected.Serum IL-9,IL-10 and TGF-β1 levels were determined by enzyme-linked immunosorbent assay（ELlSA）.Results The IL-9 levels in the serum of slight/moderate,severe and liver cirrhosis CHB patients were（319.96±367.26）,（308.47±402.77）and（432.49±591.65）ng/L,respectively;the IL-10 levels in these three CHB groups were（95.73±107.93）,（69.52±66.49）and（111.41±113.41）ng/L,respectively;the TGF-β1 were（42.40±56.57）,（138.85±178.55）and（135.32±179.44）μg/L,respectively.The levels of IL-9,IL-10,and TGF-β1 in the CHB group were significantly higher than that in the healthy controls[（139.52±193.44）ng/L,（29.73±21.27）ng/L and（5.30±14.51）μg/L,respectively].Different serum levels of IL-9 and IL-10 were detected in patients with different loads of HBV DNA;but there were no correlations between levels of IL-9,IL-10 and TGF-β1 and HBeAg status.IL-9 level showed strong positive correlation with IL-10（r=0.932,P0.001）,but weak positive correlation with TGF-β1（r=0.227,P=0.014）.IL-10 was slightly correlated with TGF-β1（r=0.217,P=0.018）.Conclusions IL-9,IL-10 and TGF-β1 might be involved in the pathogenesis of CHB.IL-9 and IL-10 may play an important role in anti-inflammation,and their increased levels may be related to the chronic infection status of CHB patients.

关 键 词：慢性乙型肝炎 IL-9 IL-10 TGF-Β1 

分 类 号：R512.62[医药卫生—内科学]