广东省低血凝滴度甲型H1N1流感病毒HA基因变异特征  

Genetic variance of low hemagglutinin-titer strains of pandemic influenza A(H1N1) 2009 virus isolated in Guangdong,China

作　　者：张欣[1] 倪汉忠[1] 邹丽容[1] 钟豪杰[1] 周杰[1] 朱晓兰[1] 杨芬[1] 管大伟[1] 肖红[1] 柯昌文[1] 武婕[1] 林锦炎[1] 

机构地区：[1]广东省疾病预防控制中心,广东广州510300

出　　处：《中国病毒病杂志》2012年第4期305-309,共5页Chinese Journal of Viral Diseases

摘　　要：目的了解2009-2011年流感季节广东省低血凝滴度甲型流感病毒(H1N1)pdm09分离株的HA基因变异特征。方法比较2009-2010和2010-2011年流感季节A(H1N1)pdm09分离株血凝滴度构成比。选取广东甲型H1N1流感病毒90株,提取病毒RNA,经RT-PCR反应扩增HA1并测序,测定的序列用生物信息软件分析,与GenBank中相关序列比较,并对推导的编码氨基酸序列进行进化分析。结果 2010-2011年流感季节,低血凝滴度分离株的比例明显增多。HA基因遗传进化分析表明,低血凝滴度分离株均在同一个分支,在氨基酸序列第197位发生了氨基酸替换(A197T)。结论 HA的第197位氨基酸变异(A197T)可能是造成低血凝滴度的重要原因。Objective To investigate the genetic variances of low hemagglutinin（HA）-titer strains of pandemic influenza A（H1N1） 2009 virus isolated in Guangdong,China,during the 2009-2011 influenza seasons.Methods The HA titers of influenza HA（H1N1）pdm09 virus isolated during 2009-2010 and 2010-2011 seasons were compared.The HA1 gene sequence from 90 strains was analyzed using software Bioedit 7.0 and MEGA 4.0.Results The relative proportion of the low HA-titer strains of the A（H1N1）pdm09 virus markedly increased during the 2010-2011 season in Guangdong,China.All isolates with HA titers lower than 8 during 2010-2011 season were grouped into the same clade,characterized by the amino acid substitution from alanine to threonine at position 197（A197T）.Conclusions A197T substitution in the HA protein may play a critical role for the reduced affinity of HA to erythrocytes.

关 键 词：甲型H1N1流感病毒 血凝 血凝素基因 受体结合位点 

分 类 号：R440[医药卫生—诊断学]