白僵菌和绿僵菌不同菌株对橙斑白条天牛幼虫的致病力  

Pathogenicity of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against larvae of Batocera davidis

作　　者：童文钢 

机构地区：[1]连城县森林病虫害防治检疫站,福建连城366200

出　　处：《福建林学院学报》2012年第3期263-267,共5页Journal of Fujian College of Forestry

基　　金：福建省科技厅重点科技资助项目(2004N013);南平市科技局科技基金资助项目(N2005Z12)

摘　　要：橙斑白条天牛是福建省杨树的一种新害虫,应用不同球孢白僵菌(Beauveria bassiana)和金龟子绿僵菌(Metarhizium anisopliae)菌株对橙斑白条天牛幼虫进行致病力测定,旨在筛选出感染该虫的高致病力菌株,为生物防治提供新的资源。结果表明,接种后,该天牛幼虫的累积死亡率随时间的延长而逐渐增高,接种绿僵菌Ma1291-2和Ma1775菌株14 d后,幼虫的校正死亡率分别为91.69%和86.50%;接种白僵菌BbDp-06菌株14 d后,幼虫校正死亡率达71.05%。绿僵菌Ma1291-2和Ma1775菌株对该幼虫的LT50分别为5.73和5.39 d,白僵菌BbDp-06菌株的LT50为8.02 d。Ma1291-2菌株对该幼虫的LC50为3.82×104孢子.mL-1,白僵菌BbDp-06菌株的LC50为3.88×104孢子.mL-1。说明绿僵菌对该幼虫的致病力较强,尤其是Ma1291-2和Ma1775菌株,在橙斑白条天牛的生物防治中将有较好的应用价值。Batocera davidis Deyrolle is a new pest of poplar in Fujian.In order to screen entomopathogenic fungi strains with high virulence against B.davidis larvae,and bioassay evaluation of B.bassiana and M.anisopliae against B.davidis larvae was carried out through dipping in spore suspension.The results showed that the cumulative corrected mortality of B.davidis larvae was gradually increased with time after inoculated with B.bassiana and M.anisopliae.The corrected mortality of B.davidis larvae on the 14th day after inoculated with Ma1291-2 and Ma1775 were 91.69% and 86.50%,respectively,and the mortality was 71.05% on the 14th day after inoculated with BbDp-06.The muscardine cadaver rate of larvae inoculated with M.anisopliae was significantly higher than that inoculated with B.bassiana.While LT50 value of the two M.anisopliae strains,Ma1291-2 and Ma1775 were 5.73 and 5.39 days,respectively,and LT50 value of B.bassiana BbDp-06 was 8.02 days.LC50 value of two strains,Ma1291-2 and BbDp-06 were 3.82×104 spore · mL-1 and 3.88×104 spore · mL-1,respectively.The results of bioassay showed that Ma1291-2 and Ma1775 strains had higher virulence to B.davidis larvae than BbDp-06 strain.A separate field bioassay suggested that the 2 strains,Ma1291-2 and Ma1775 could be used to control B.davidis larvae.

关 键 词：球孢白僵菌 金龟子绿僵菌 杨树 橙斑白条天牛 致病力 

分 类 号：Q965.8[生物学—昆虫学]