溃结2号方对溃疡性结肠炎大鼠溃疡组织中IL-1β及IL-10 mRNA表达的影响  

Effect of No.2 Kuijie Recipe on IL-1β and IL-10 mRNA Expression in Colonic Mucosa of Ulcerative Colitis Rats

作　　者：梁兴伦 吴闯[2] 姜在龙[3] 金炜[2] 吴炯[2] 李盈[2] 王振宜[2] 

机构地区：[1]同济大学医学院附属杨浦医院,上海200090 [2]上海中医药大学附属岳阳中西医结合医院肛肠科,上海200437 [3]浙江中医药大学第二附属医院肛肠科,浙江杭州310005

出　　处：《广州中医药大学学报》2012年第4期397-401,488,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：上海市教委课题资助项目(编号:09YZ127)

摘　　要：【目的】观察溃结2号方对实验性溃疡性结肠炎(ulcerative colitis,UC)模型大鼠结肠溃疡组织白细胞介素1β(IL-1β)、白细胞介素10(IL-10)mRNA表达的影响。【方法】采用2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acidTNBS)及免疫诱导法复制UC大鼠模型,将造模成功的36只大鼠随机分为模型组、中药组(剂量为16.76 g.kg-1.d-1)、柳氨磺吡啶(SASP)组(剂量为0.206 g.kg-1.d-1),每组各12只。观察各组大鼠治疗14 d及21 d后结肠组织的病理改变,采用逆转录—聚合酶链反应(RT-PCR)方法检测大鼠结肠组织中IL-1βmRNA、IL-10 mRNA的表达。【结果】给药14 d时,中药组大鼠结肠组织中IL-1βmRNA、IL-10 mRNA表达与模型组及SASP组比较差异均无统计学意义(P>0.05);给药21 d时,中药组大鼠结肠组织中IL-1βmRNA表达量显著低于模型组(P<0.01),IL-10 mRNA表达显著高于模型组(P<0.01)。中药组对UC大鼠结肠组织病理损伤具有修复作用,且效果优于SASP组。【结论】溃结2号方对UC的治疗作用与其能调节结肠组织IL-1βmRNA、IL-10 mRNA表达水平有关。Objective To observe the effects of No. 2 Kuijie Recipe （KR2） on IL-1β and IL-10 mRNA expression in the colonic mucosa of ulcerative colitis （UC） rats. Methods Thirty-six SD rats were randomly divided into three groups, model group, KR2 （16. 76 gk·kg^-1·d^-1） group, and salicylazosulfapyridine （SASP, 0. 206 g·kg^-1·d^-1） group, 12 rats in each group. Rat models of UC were induced with 2, 4, 6-trinitrobenzene sulfonic acid （TNBS） and by immune induction method. After treatment for 14 and 21 days, the histopathological changes of colonic mucosa were observed, and IL-1β and IL-10 mRNA expression levels in the colonic mucosa were detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results After treatment for 14 days, IL-1β and IL-10 mRNA expression in KR2 group did not differ from that in the model group and SASP group （P 〉0. 05）. After treatment for 21 days, IL-1β mRNA expression level was lower and IL-10 mRNA expression level was higher in KR2 group than those in the model group （P 〈0. 01 ）. The effect on relieving the injury of colonic mucosa of KR2 group was superior to that in SASP group. Conclusion The therapeutic mechanism of KR2 for the treatment of UC is probably related with the regulation of IL-1β and IL-10 mRNA expression levels in the colonic mucosa.

关 键 词：溃结2号方/药理学 溃疡性结肠炎/中药疗法 结肠/病理学 疾病模型 动物 大鼠 

分 类 号：R284.5[医药卫生—中药学]