大熊猫肠道大肠杆菌的分离、鉴定及其耐药性分析  

Isolation and Drug Resistance Analysis on Escherichia coli in Intestines from Giant Pandas

作　　者：李蓓[1] 李旭林[1] 郭丽娟[1] 龙梅[1] 郭放[1] 何雪梅[1] 罗燕[1] 邹立扣[1] 

机构地区：[1]四川农业大学都江堰校区微生物学实验室,森林资源保护与利用实验室,四川都江堰611830

出　　处：《西南农业学报》2012年第3期1109-1113,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省教育厅重点项目(10ZA055)

摘　　要：本实验分离大熊猫粪便样品中大肠杆菌,利用形态学和分子生物学方法鉴定菌株,采用药敏纸片扩散法(Kirby-Bauer)进行常用抗生素耐药性试验分析。结果表明,从抗生素大类来比较,耐药率依次为:四环素类(55.6%)>青霉素类(26.7%)>磺胺类(18.9%)>喹诺酮类(10%)>头孢类(7.8%)>氨基糖苷类(1.1%)>单环内酰胺类(0)、碳青霉烯类(0)。大肠杆菌对四环素的耐药率最高,达到55.6%;对头孢曲松、头孢他啶、氨曲南、亚胺配能及美罗配能等敏感,耐药率为0。其它抗菌药物耐药率依次是:阿莫西林(26.7%)>磺胺复合物(18.9%)>氨苄西林/舒巴坦(17.8%)>复方新诺明(11.1%)>萘啶酸(10%)>头孢噻吩(7.8%)>阿莫西林/克拉维酸(3.3%)>庆大霉素(2.2%),共产生了33种耐药谱,大部分菌株至少耐1种药物,TET为优势耐药谱(18/90),其次为TET、AML(10/90),多重耐药较普遍(40/90),个别菌株耐药谱达到6种,此研究为大熊猫肠道疾病的合理、科学治疗奠定了基础。Escherichia coli was isolated fi＇om Giant panda feces and identified by morphology and molecular biology method. Antimicrobial susceptibility were tested by the standardized Disk diffusion method （ Kirby-Baner）. Results showed that resistance rates of tetracycline, pen- icillin, sulfonamidos, quinolanes, cephalospofins, aminoglycosides, monobactam and earbapenem were 55.6 % ,26.7 %, 18.9 %, 12.2 % ,7.8 % ,1.1% ,0,0,respectively. The E. coli strains were the most resistant to tetracycline（55.6 % ）, followed by amoxieillin（26.7 % ）, sulfanilamides complex （ 18.9 % ）, ampicillin/sulbactam （ 13.3 % ）, trimethoprim= sulfamethoxazole （ 11.1% ）, nalidixic acid（ 10 % ）, eefalotin（7.8 % ）, amoxicillin/clavulanic acid（3.3 % ） and gentamicin（2.2 % ） ,respectively. Besides,all of the isolates were sen- sitive to cezftriaxone, ceftasidime, aztreonam, imipenem and meropenem. There appeared 33 resistance spectrums. Most of the stains （40/90） were resistant to at least one drug. The TET was the most popular drug resistant spectrum（ 18/90）, fo/lowe by Tet/AML（10/90） ,and sever- a/strains were resistant to six different drugs. The research provided the base for treating intestinal tract diseases in giant pandas.

关 键 词：大熊猫 大肠杆菌 分离 耐药性 

分 类 号：Q954[生物学—动物学] Q93