原发性免疫缺陷病患者B淋巴母细胞系的建立及其核型检测  

Establishment and karyotype detection of Epstein-Barr virus-transformed B lymphoblastoid cell lines from patients with primary immunodeficiency diseases

作　　者：姜昱竹[1] 胡雪梅[1] 邢建琴[2] 王跃嗣[1] 刘现兵[1] 郭春凤[2] 

机构地区：[1]滨州医学院烟台校区,山东烟台264003 [2]威海市文登中心医院

出　　处：《山东医药》2012年第27期41-43,共3页Shandong Medical Journal

基　　金：滨州医学院科研启动基金资助项目(BY2011KYQD04)

摘　　要：目的体外建立原发性免疫缺陷病(PIDD)患者永生化B淋巴母细胞系(B-LCL),为进一步开展PIDD相关研究提供资源。方法自PIDD患者外周血中分离单个核细胞,采用EB病毒感染并加入环胞菌素A抑制T淋巴细胞活性的方法建立B-LCL,7 d左右倒置显微镜下观察转化细胞的形态,并进行染色体核型分析。结果转化后的B淋巴母细胞体积增大,增生的淋巴细胞多聚集成团;已建立的永生化细胞能够成功冻存、复苏及传代;转化前后细胞染色体核型无明显改变。结论本实验成功建立了PIDD患者永生化的B-LCL,为进一步开展PIDD的病因学研究及诊断、治疗奠定了基础。Objective To establish the immortalized B lymphoblastoid cell lines （B-LCL） from patients with primary immunodeficiency diseases （PIDD） in vitro, so to provide related resources for further study of PIDD. Methods The pe-ripheral blood mononuclear cells （PBMCs） were isolated from the patients with PIDD by Ficoll density gradient centrifuga-tion method, then were incubated with Epstein-Barr virus （EBV） in the presence of cyclosporin A （CsA） to establish B- LCL by inhibiting the activity of T lymphocytes. Morphology of the transformed cells was observed under inverted micro-scope. Karyotype analysis of PBMCs and B-LCL was performed. Results The cellular volume of those transformed cells was enlarged and there was increasing development of cell aggregates ; the immortalized B-LCLs could be cultured and prop-agated for a long time in vitro with no significant change in karyotype. Conclusion Immortalized B-LCL from patients with PIDD is successfully established in this study, which provides a basis for the etiological research, diagnosis and treatment of PIDD.

关 键 词：原发性免疫缺陷病 EB病毒 永生化 B淋巴母细胞系 

分 类 号：R392.2[医药卫生—免疫学]