桃花芽休眠解除相关上调表达基因的cDNA-AFLP分析  

Identification of differentially expressed up-regulated genes by cDNA-AFLP approach during dormancy release in peach flower buds

作　　者：罗静[1,2] 张巧莲[3] 武峥 漆巨容 谢永红 王武 王进 刘正富 

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]重庆农业科学院果树研究所,重庆江津402260 [3]许昌职业技术学院园艺园林系,河南许昌461000

出　　处：《果树学报》2012年第4期557-562,共6页Journal of Fruit Science

基　　金：重庆市自然科学基金计划项目(CSTC;2009BB1247)

摘　　要：【目的】桃在冬天为了避免低温伤害而停止生长的发育过程称之为自然休眠。为研究桃花芽休眠解除的分子遗传机制,筛选休眠相关基因。【方法】以中需冷量桃品种‘满天红’为试材,采用cDNA-AFLP技术鉴定花芽内差异表达的基因,并对上调基因进行功能预测。【结果】12对引物共扩增出525条差异TDFs(transcripts-derived fragment),其中与休眠解除相关的上调TDFs 14条,下调的TDFs 18条。对休眠解除后上调的14个差异条带进行克隆测序,经Blast显示其中10个基因分别与泛素连接酶E3、植物组蛋白H3、核糖体蛋白L34、Prohibitin-2蛋白以及半乳糖基转移酶等基因同源性较高。【结论】筛选到的休眠相关基因功能涉及代谢、逆境响应、细胞分化及信号转导,这些基因有助于我们更好的解析桃花芽休眠的机制。[Objective] Peach （Prunus persica [L.] Batsch） ceases growth and becomes dormant during winter to elude the detrimental effect of exposure to low temperatures. This state has been designated endodormancy. To better understand the genetic control of flower bud dormancy in peach and select the related gene, [Method] We identified differentially expressed genes during dormancy progression in flower buds from medium chilling requirement peach variety ＇Mantianhong＇ by using cDNA-AFLP. [Result] Twelve primer combinations were used for selective amplification. Five hunderd and twenty-five TDFs （transcripts-derived fragments） were obtained for their differential expression during dormancy release. Among the 525 TDFs, 14 TDFs were up-regulated and 18 TDFs were suppressed. Fourteen up-regulated TDFs were cloned and sequenced. Ten TDFs presented high similarity to known genes which encoded E3 ubiquitin-protein ligase CIP8, Histone H3.3, 60S ribosomal protein L34, Prohibitin-2, and Galactinol- sucrose galactosyhransferase 2. [Conclusion] The TDFs whose functions are involved in metabolism, stress response, cell structure and plant growth and signal transit according to their primary BLAST match could help us to understand the mechanism of bud dormancy in peach.

关 键 词：桃 休眠 差异表达基因 cDNA—AFLP 

分 类 号：S662.1[农业科学—果树学]