抗生素对中华猕猴桃外植体再生的影响  

Effects of antibiotics on explant regeneration of Actinidia chinensis

作　　者：尚霄丽[1,2] 谭彬[1] 李继东[1] 张建鹏[2] 冯建灿[1] 

机构地区：[1]河南农业大学,郑州450002 [2]濮阳职业技术学院,河南濮阳457000

出　　处：《果树学报》2012年第4期611-615,共5页Journal of Fruit Science

基　　金：河南省杰出青年基金项目(084100410021)

摘　　要：【目的】为确定中华猕猴桃遗传转化中适宜的抗生素种类和浓度,【方法】以中华猕猴桃‘伏牛95-2’叶片为外植体,研究了抗生素对外植体再生的影响。【结果】抗生素敏感性测定发现,当卡那霉素质量浓度为20 mg.L-1时,愈伤组织及不定芽分化率均为0,因此,20 mg.L-1卡那霉素为适宜的筛选浓度;头孢霉素和羧苄青霉素对中华猕猴桃‘伏牛95-2’叶片分化均有抑制作用,但头孢霉素对叶片分化的抑制作用比羧苄青霉素小,结合抑菌试验,将浓度400 mg.L-1头孢霉素确定为适宜于中华猕猴桃‘伏牛95-2’叶片遗传转化的抑菌抗生素。【结论】卡那霉素20 mg.L-1和头孢霉素400 mg.L-1为中华猕猴桃‘伏牛95-2’遗传转化适宜的抗生素及质量浓度。[Objective] The study is to screen the suitable type and concentration of antibiotics for the transformation of A ctinidia chine ns is. [ Method ] Effects of antibiotics on explant regeneration were studied using leaves of A ctinidia chinensis ‘Funiu 95-2＇ as explants. [Result] The results showed that differentiation rate of callus and adventitious shoots was zero when 20 rag. L^（-1） kanamycin was added into the medium, so the optimal kanamycin concentration for the selection of transformants was 20 mg·L-1 based on the antibiotic sensitivity measurement. Differentiation of adventitious shoots was inhibited by adding Cefotaxime and Carbenicillin into the medium, while inhibition effect of Carbenicillin was stronger than that of Cefotaxime. 400 rag. L^（-1） Cefotaxime was optimal for genetic transformation by Agrobacteriumin in Actinidia chinensis ‘Funiu 95-2＇ [Conclusion] The studies suggested that 20 mg.L^（-1） Kanamycin and 400 rag.L^（-1） Cefotaxime were suitable for the transformation of Actinidia chinensis ‘Funiu 95-2＇.

关 键 词：中华猕猴桃'伏牛95—2’ 抗生素 外植体 

分 类 号：S663.4[农业科学—果树学]