玉米纹枯病菌UP-PCR体系的建立及遗传多样性的分析  

Construction of UP-PCR System and Genetic Diversity Analysis of Rhizoctonia solani

作　　者：周晓锟[1] 高增贵[1] 张秀霞[1] 张硕[1] 李忠洲[1] 高金欣[1] 

机构地区：[1]沈阳农业大学植物免疫研究所,沈阳110161

出　　处：《沈阳农业大学学报》2012年第1期33-38,共6页Journal of Shenyang Agricultural University

基　　金：国家粮食丰产科技工程项目(2011BAD16B12);现代农业产业技术体系项目(CARS-02);辽宁省教育厅重点实验室项目(LS2010149)

摘　　要：对UP-PCR(Universally Primed PCR)反应中的一些重要参数进行了优化,建立适合于玉米纹枯病菌的UP-PCR反应体系。对分离自辽宁、吉林、黑龙江等玉米主产区的22个菌株和16个标准菌株进行遗传多态性分析,筛选出7个扩增多态性好且稳定的通用引物,共扩增出105条带,其中多态性条带102条,占总条带数的97.1%。当相似系数为0.685时,可将38株镰孢菌菌株划分为10个类群,其中所有AG1-IA菌株聚为一个类群。聚类分析结果表明,供试菌株的遗传多样性与其所属融合群有着明显的相关性,而与其地理来源之间无明显的相关性。Abstract： Essential factors affecting the results of UP-PCR （Universally Primed PCR） analysis were tested and compared. An optimal reaction system suited for UP-PCR in genetic diversity was established. Seven polymorphic primers were selected to study the genetic diversity among 16 standard-isolates and 22 tester isolates coming from the major corn planting areas in Northeastern China. The results showed that 105 amplified loci were detected, of which 102 （97.1%） were polymorphic. The isolates were clustered into 10 groups at the similar coefficient 0.685 by cluster analysis, of which, all the isolates belong to AGI-IA were clustered into one group. Cluster analysis showed that there was a clear correlation between genelic diversity and anastomosis group, but no significant correlation between ~enetie diversity and ~eozraDhic source.

关 键 词：玉米纹枯病菌 融合群 UP—PCR 遗传多样性 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]