黄芪多糖-枯草芽孢杆菌合生元菌液的研制  

Preparation of Bacillus subtilis-astragalus Complex Biological Reagent

作　　者：文宇婷[1] 边连全[1] 杜欣[1] 潘树德[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161

出　　处：《沈阳农业大学学报》2012年第1期98-101,共4页Journal of Shenyang Agricultural University

基　　金：“十一五”国家科技支撑项目(2007BAD71B03);“十二五”国家科技支撑计划项目(2011BAD28B01-02)

摘　　要：研究黄芪多糖-枯草芽孢杆菌合生元菌液制备过程中,黄芪多糖的最适添加量以及合生元菌液的最适培养时间。将活化后的枯草芽孢杆菌ACCC11025菌液以1%的接种量分别加入含黄芪多糖浓度为0,0.5,1,1.5,2,2.5,3,6,10mg.mL-1的液体培养基中,分别置37℃、120r.min-1以及32℃、120r.min-1培养箱中培养,每隔0.5h检测菌液OD值。结果表明:添加浓度为1.5mg.mL-1黄芪多糖的培养基中,枯草芽孢杆菌ACCC11025菌液浓度达到最高,分别比对照组和10mg.L-1组提高13.8%和148.8%(p<0.05);在5.5h代时最小,为1.22h,黄芪多糖-枯草芽孢杆菌合生元黄芪多糖最适添加量为1.5mg.mL-1,最适培养时间为5.5h。Abstract： This experiment was conducted to study the preparation of Bacillus subtilis-astragolus complex biological reagent,the optimal ratio of the APS and Bacillus subtilis and the best training time. Inoculate the activated Bacillus subtilis into the liquid medium, which contained different concentrations of APS. The concentrations of APS were 0, 0.5, 1, 1.5, 2, 2.5, 3, 6, 10mg.mL-1 respectively. Then train them in the 37°C, 120r-min-1 incubator. Measurement was made every 0.5h at 540nm absorbance. The results showed that the number of Bacillus subtilis in the liquid medium with 1.5mg.mL-1 concentration of APS was the highest, and the effect was significantly （p〈0.05）.The best training time was 5.5h. These resuhs indicated that the optimum dosage of APS for Bacillus subtilis ACCC11025 is 1.Smg＇mL-1, and the optimum incubation time is 5.5h.

关 键 词：黄芪多糖 枯草芽孢杆菌 合生元 

分 类 号：S816.71[农业科学—饲料科学]